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Main conclusion <p>Chalcone isomerase (<i>BraCHI</i>, <i>BraA03g059660.3C</i>) is the candidate gene controlling purple leaf coloration in non-heading Chinese cabbage. A 10-bp deletion in its promoter enhances gene expression in purple plants, likely by disrupting MYB transcription factor binding, leading to anthocyanin accumulation.</p> Abstract <p>Leaf color is a critical trait influencing the commercial and nutritional value of leafy vegetables, with purple-leafed varieties prized for their high anthocyanin content. In this study, we investigated the genetic basis of purple leaf coloration in non-heading Chinese cabbage (<i>Brassica rapa</i>). Using a recombinant inbred line (RIL) population derived from a cross between purple-leafed S45P and green-leafed S45G lines, bulked segregant analysis sequencing (BSA-seq) and fine mapping were performed. The analysis identified <i>BraP2</i>, a locus on chromosome A03 associated with purple leaf coloration. Within the 65.31&#xa0;kb candidate region, <i>BraA03g059660.3C</i>, encoding chalcone isomerase (<i>CHI</i>), was identified as the strongest candidate gene. Quantitative real-time PCR (qRT-PCR) revealed significantly higher expression of <i>BraA03g059660.3C</i> in purple-leafed S45P plants compared to green-leafed S45G plants. Further sequence analysis uncovered a 10-bp deletion in the promoter region of <i>BraA03g059660.3C</i> in S45P plants. This deletion likely disrupts a MYB transcription factor binding site, enhancing gene expression and promoting anthocyanin accumulation. Our findings demonstrate that <i>BraA03g059660.3C</i> plays a pivotal role in controlling purple leaf coloration in non-heading Chinese cabbage. This discovery advances the understanding of anthocyanin biosynthesis regulation and provides valuable genetic resources for breeding <i>Brassica</i> crops with improved esthetic and nutritional qualities.</p>

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Identification and characterization of a key gene controlling purple leaf coloration in non-heading Chinese cabbage (Brassica rapa)

  • 期刊论文

  • 发表日期：

    2025年3月6日

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  • Jia Si,
  • Xiaoqing Zhou,
  • Xinyu Chen,
  • Huilin Ming,
  • Hanqiang Liu,
  • Maixia Hui

摘要

Main conclusion

Chalcone isomerase (BraCHI, BraA03g059660.3C) is the candidate gene controlling purple leaf coloration in non-heading Chinese cabbage. A 10-bp deletion in its promoter enhances gene expression in purple plants, likely by disrupting MYB transcription factor binding, leading to anthocyanin accumulation.

Abstract

Leaf color is a critical trait influencing the commercial and nutritional value of leafy vegetables, with purple-leafed varieties prized for their high anthocyanin content. In this study, we investigated the genetic basis of purple leaf coloration in non-heading Chinese cabbage (Brassica rapa). Using a recombinant inbred line (RIL) population derived from a cross between purple-leafed S45P and green-leafed S45G lines, bulked segregant analysis sequencing (BSA-seq) and fine mapping were performed. The analysis identified BraP2, a locus on chromosome A03 associated with purple leaf coloration. Within the 65.31 kb candidate region, BraA03g059660.3C, encoding chalcone isomerase (CHI), was identified as the strongest candidate gene. Quantitative real-time PCR (qRT-PCR) revealed significantly higher expression of BraA03g059660.3C in purple-leafed S45P plants compared to green-leafed S45G plants. Further sequence analysis uncovered a 10-bp deletion in the promoter region of BraA03g059660.3C in S45P plants. This deletion likely disrupts a MYB transcription factor binding site, enhancing gene expression and promoting anthocyanin accumulation. Our findings demonstrate that BraA03g059660.3C plays a pivotal role in controlling purple leaf coloration in non-heading Chinese cabbage. This discovery advances the understanding of anthocyanin biosynthesis regulation and provides valuable genetic resources for breeding Brassica crops with improved esthetic and nutritional qualities.