Introduction <p>The presence of sex differences in various vascular assessments in younger to middle-aged adults is inconsistent in the literature.</p> Purpose <p>Determine whether sex differences are present across four different indices of vascular health.</p> Methods <p>Healthy, recreationally active premenopausal females (<i>N</i> = 30) and similarly aged males (<i>N</i> = 30) aged 18–54 yrs performed four vascular assessments. Assessments included resting augmentation index (AIx%), carotid-femoral pulse wave velocity (cf-PWV, ms^− 1), and simultaneous digital thermal monitoring (temperature rebound; TR%) and near-infrared spectroscopy-based microvascular reactivity (10s reperfusion slope; slope 2, %s^− 1).</p> Results <p>AIx (<i>p</i> = 0.006) was significantly lower in males vs. females. Sex differences were eliminated after covarying for mean adjusted height (8.9 ± 9.1% vs. 16.8 ± 11.9% in males and females, respectively; <i>p</i> = 0.360) or when examining a subset of individuals (<i>N</i> = 14 males and <i>N</i> = 14 females) with similar body height (13.0 ± 8.7% vs. 16.4 ± 7.9% in males and females, respectively; <i>p</i> = 0.286). Cf-PWV was significantly higher in males (6.0 ± 0.8 ms^− 1) vs. females (5.2 ± 0.7 ms^− 1; <i>p</i> &lt; 0.001). We found no differences between males and females in TR (1.2 ± 1.1% vs. 1.1 ± 0.8%, respectively; <i>p</i> = 0.771), or StO₂ slope 2 (2.2 ± 0.6%s^− 1 vs. 1.9 ± 0.9%s^− 1, respectively; <i>p</i> = 0.254).</p> Conclusions <p>After accounting for confounding variables, differences in cf-PWV were detected between sexes, while sex differences in other assessments of vascular health were not observed.</p>