Purpose <p>This study aimed to investigate the effects of static stretching exercise (SSE) on arterial stiffness following high-intensity resistance exercise (RE).</p> Methods <p>In a randomized controlled crossover trial, 12 healthy young men performed either SSE or seated rest (CON) following high-intensity RE. The participants completed five sets of five repetitions at 80% of one-repetition maximum (1RM) for the bench press and five sets of 10 repetitions at 70% of 1RM for biceps curl. Brachial-ankle pulse wave velocity (baPWV), carotid arterial compliance, and stiffness parameter β were measured at baseline, immediately after RE, and immediately after SSE or CON, as well as at 30&#xa0;min (P30) and 60&#xa0;min (P60) following the completion of SSE or CON.</p> Results <p>At baseline, no significant differences were observed in baPWV, carotid arterial compliance, or stiffness parameter β. Both baPWV and stiffness parameter β significantly increased immediately after RE in both trials compared to baseline. In the SSE trial, baPWV was lower at P30 and P60 than in the CON trial. Similarly, stiffness parameter β was lower immediately after SSE, and at P30 and P60 in the SSE trial than in the CON trial. Carotid arterial compliance decreased immediately after RE when compared to baseline in both trials; however, it was higher immediately after SSE, and at P30 and P60 in the SSE trial compared with the CON trial.</p> Conclusion <p>SSE following high-intensity RE mitigates the increase in arterial stiffness in healthy young men.</p>