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Purpose <p>Diabetes mellitus (DM) is associated with increased oxidative stress, which contributes to systemic dysfunction and respiratory muscle impairment. This study aimed to evaluate the effects of respiratory muscle training (RMT) on oxidative stress and antioxidant activity in diabetic rats induced by a high-fat diet and low-dose streptozotocin.</p> Methods <p>Male Wistar rats (<i>n</i> = 30) were divided into four groups: sedentary-sham (Sed-Sham, <i>n</i> = 8), respiratory muscle trained-sham (RMT-Sham, <i>n</i> = 8), sedentary-DM (Sed-DM, <i>n</i> = 7), and respiratory muscle trained-DM (RMT-DM, <i>n</i> = 7). DM was induced using a high-energy diet followed by a low-dose streptozotocin injection. The RMT protocol consisted of 30&#xa0;min/day, 5&#xa0;days/week, for 6&#xa0;weeks, whereas the sedentary animals did not exercise. Oxidative stress was assessed by measuring thiobarbituric acid reactive substances (TBARS) and dichlorofluorescein (DCF) levels, while antioxidant capacity was evaluated through superoxide dismutase (SOD) activity and non-protein thiol (NPSH) levels in plasma and selected tissues.</p> Results <p>In DM rats, RMT significantly reduced TBARS levels in plasma and gastrocnemius muscle, indicating a decrease in lipid peroxidation. Furthermore, RMT partially restored antioxidant capacity, as evidenced by increased SOD activity in the lungs and elevated NPSH levels in the liver. In Sham rats, RMT also demonstrated beneficial effects, reducing TBARS levels in the gastrocnemius muscle and enhancing SOD activity in the kidneys.</p> Conclusion <p>RMT attenuated oxidative stress in DM rats by reducing lipid peroxidation and enhancing antioxidant defenses. These findings suggest RMT as a potential non-pharmacological strategy to improve oxidative balance in rats with diabetes.</p>

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Respiratory muscle training attenuates oxidative stress and improves antioxidant activity in diabetic rats induced by high-fat diet and low-dose streptozotocin

  • 期刊论文

  • 发表日期：

    2025年8月31日

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  • R. B. Jaenisch,
  • C. C. F. Silva,
  • L. S. Tonetto,
  • N. Gonzatti,
  • C. G. Guex,
  • G. O. Puntel,
  • A. M. V. Silva,
  • L. U. Signori,
  • P. Dal Lago,
  • L. F. Bauermann,
  • M. E. Trevisan

摘要

Purpose

Diabetes mellitus (DM) is associated with increased oxidative stress, which contributes to systemic dysfunction and respiratory muscle impairment. This study aimed to evaluate the effects of respiratory muscle training (RMT) on oxidative stress and antioxidant activity in diabetic rats induced by a high-fat diet and low-dose streptozotocin.

Methods

Male Wistar rats (n = 30) were divided into four groups: sedentary-sham (Sed-Sham, n = 8), respiratory muscle trained-sham (RMT-Sham, n = 8), sedentary-DM (Sed-DM, n = 7), and respiratory muscle trained-DM (RMT-DM, n = 7). DM was induced using a high-energy diet followed by a low-dose streptozotocin injection. The RMT protocol consisted of 30 min/day, 5 days/week, for 6 weeks, whereas the sedentary animals did not exercise. Oxidative stress was assessed by measuring thiobarbituric acid reactive substances (TBARS) and dichlorofluorescein (DCF) levels, while antioxidant capacity was evaluated through superoxide dismutase (SOD) activity and non-protein thiol (NPSH) levels in plasma and selected tissues.

Results

In DM rats, RMT significantly reduced TBARS levels in plasma and gastrocnemius muscle, indicating a decrease in lipid peroxidation. Furthermore, RMT partially restored antioxidant capacity, as evidenced by increased SOD activity in the lungs and elevated NPSH levels in the liver. In Sham rats, RMT also demonstrated beneficial effects, reducing TBARS levels in the gastrocnemius muscle and enhancing SOD activity in the kidneys.

Conclusion

RMT attenuated oxidative stress in DM rats by reducing lipid peroxidation and enhancing antioxidant defenses. These findings suggest RMT as a potential non-pharmacological strategy to improve oxidative balance in rats with diabetes.