<p>The present research investigates the effects of activation energy and electroosmosis on the peristaltic transport of a pseudoplastic nanofluid flowing through an asymmetric, flexible microchannel. The analysis incorporates crucial factors, such as thermal radiation, magnetic field, thermophoresis, and Brownian motion. The governing mathematical model is simplified using the lubrication approximation. The resulting system of equations is then numerically solved using NDSolve in Mathematica. The impact of key fluid properties on electroosmotic flow is examined through graphical analysis. Results demonstrate that nanofluid velocity decreases with increasing Debye–Hückel parameter. Furthermore, fluid flow is reduced with higher pseudoplastic fluid parameters, while nanoparticle concentration diminishes with increasing temperature ratio parameters. Nanofluid temperature increases with an enhancement in the thermophoresis parameter.</p>