Scholarlink

Background <p>In assisted reproduction, poor ovarian response to stimulation negatively affects oocyte yield and is influenced by genetic factors.</p> Objective <p>This study aimed to quantify the mRNA expression of key growth markers (BMP15, GDF9, OCT4, and FGFR2) in ovarian tissue according to the developmental stages of the follicle.</p> Methods <p>Samples were collected from ovarian tissue. Gene expression levels were analyzed using RT-qPCR. In addition, ELISA was used to measure the concentrations of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD), along with reactive oxygen species (ROS) and malondialdehyde (MDA) as oxidative stress markers.</p> Results <p>OCT4 expression was similar in preantral and small follicles but significantly upregulated in medium and large follicles. GDF9 expression and SOD activity were highest in small follicles (<i>P</i> &lt; 0.05). BMP15 levels were significantly elevated in small and medium follicles compared to preantral follicles but remained unchanged in large follicles (<i>P</i> &lt; 0.05). FGFR2 expression increased progressively with follicle size (<i>P</i> &lt; 0.05). GPx activity was directly proportional to follicle size, with the lowest levels in preantral follicles. Conversely, ROS, MDA, and CAT concentrations decreased as follicle size increased.</p> Conclusion <p>These findings provide insights into the molecular regulation of follicular development in buffalo, which could aid in improving reproductive efficiency in assisted reproduction programs.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Expression of growth factors in buffalo ovarian tissue across different follicular developmental stages

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年6月21日

查看全文
  • Seham Samir Soliman,
  • Marwa El-Sheikh,
  • Dalia A. Taha,
  • Karima A. Hamed,
  • Wagdy K. B. Khalil

摘要

Background

In assisted reproduction, poor ovarian response to stimulation negatively affects oocyte yield and is influenced by genetic factors.

Objective

This study aimed to quantify the mRNA expression of key growth markers (BMP15, GDF9, OCT4, and FGFR2) in ovarian tissue according to the developmental stages of the follicle.

Methods

Samples were collected from ovarian tissue. Gene expression levels were analyzed using RT-qPCR. In addition, ELISA was used to measure the concentrations of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD), along with reactive oxygen species (ROS) and malondialdehyde (MDA) as oxidative stress markers.

Results

OCT4 expression was similar in preantral and small follicles but significantly upregulated in medium and large follicles. GDF9 expression and SOD activity were highest in small follicles (P < 0.05). BMP15 levels were significantly elevated in small and medium follicles compared to preantral follicles but remained unchanged in large follicles (P < 0.05). FGFR2 expression increased progressively with follicle size (P < 0.05). GPx activity was directly proportional to follicle size, with the lowest levels in preantral follicles. Conversely, ROS, MDA, and CAT concentrations decreased as follicle size increased.

Conclusion

These findings provide insights into the molecular regulation of follicular development in buffalo, which could aid in improving reproductive efficiency in assisted reproduction programs.