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<p>Psoriasis is a chronic inflammatory dermatological disorder characterized by abnormal keratinocyte proliferation and dysregulation of the immune system dysregulation. Recent evidence suggests that microRNAs (miRNAs) play cricial regulatory roles in the pathogenesis of psoriasis. This study investigates the biomarker potential of microRNA-7-5p (miR-7-5p) in psoriasis and its mechanistic influence on keratinocyte-mediated inflammation. Serum from 75 psoriasis patients and 60 controls underwent miR-7-5p measurement by quantitative real-time polymerase chain reaction (qRT-PCR), with receiver operating characteristic curve analysis determining diagnostic accuracy. Interleukin-22 (IL-22)-treated (100 ng/mL) HaCaT cells served as an in vitro model to examine miR-7-5p’s functional effects via mimic/inhibitor experiments assessing proliferation and cytokine production. Statistical analysis revealed significantly upregulated miR-7-5p levels in the serum of psoriasis patients (<i>P</i> &lt; 0.001), demonstrating excellent diagnostic capability (area under the curve = 0.911). Strong positive correlations were identified between miR-7-5p expression and key inflammatory markers: IL-17&#xa0;A (<i>r</i> = 0.6666), IL-23 (<i>r</i> = 0.6059), and tumor necrosis factor-alpha (TNF-α) (<i>r</i> = 0.5789) (<i>P</i> &lt; 0.001). In IL-22-stimulated HaCaT cells, miR-7-5p mimic exacerbated proliferation and cytokine production, while its inhibitor suppressed these effects (<i>P</i> &lt; 0.001). MiR-7-5p was significantly elevated and has emerged as a promising diagnostic biomarker for psoriasis by modulating the keratinocyte inflammatory responses of keratinocyte. Targeting miR-7-5p may provide novel therapeutic strategies.</p>

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MicroRNA-7-5p is a promising diagnostic indicator for patients with psoriasis and associated with inflammatory events in keratinocytes

  • 期刊论文

  • 发表日期：

    2025年12月9日

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  • Xiaoling Zhao,
  • Yingying Xue,
  • Gaiping Wang,
  • Shixin Lei,
  • Liang Yang,
  • Min You

摘要

Psoriasis is a chronic inflammatory dermatological disorder characterized by abnormal keratinocyte proliferation and dysregulation of the immune system dysregulation. Recent evidence suggests that microRNAs (miRNAs) play cricial regulatory roles in the pathogenesis of psoriasis. This study investigates the biomarker potential of microRNA-7-5p (miR-7-5p) in psoriasis and its mechanistic influence on keratinocyte-mediated inflammation. Serum from 75 psoriasis patients and 60 controls underwent miR-7-5p measurement by quantitative real-time polymerase chain reaction (qRT-PCR), with receiver operating characteristic curve analysis determining diagnostic accuracy. Interleukin-22 (IL-22)-treated (100 ng/mL) HaCaT cells served as an in vitro model to examine miR-7-5p’s functional effects via mimic/inhibitor experiments assessing proliferation and cytokine production. Statistical analysis revealed significantly upregulated miR-7-5p levels in the serum of psoriasis patients (P < 0.001), demonstrating excellent diagnostic capability (area under the curve = 0.911). Strong positive correlations were identified between miR-7-5p expression and key inflammatory markers: IL-17 A (r = 0.6666), IL-23 (r = 0.6059), and tumor necrosis factor-alpha (TNF-α) (r = 0.5789) (P < 0.001). In IL-22-stimulated HaCaT cells, miR-7-5p mimic exacerbated proliferation and cytokine production, while its inhibitor suppressed these effects (P < 0.001). MiR-7-5p was significantly elevated and has emerged as a promising diagnostic biomarker for psoriasis by modulating the keratinocyte inflammatory responses of keratinocyte. Targeting miR-7-5p may provide novel therapeutic strategies.