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<p>Mesenchymal stem cell-derived exosomes (MSC-Exos) have been shown to protect melanocytes from oxidative injury, suggesting their potential contribution to therapeutic strategies for vitiligo. Multilineage-differentiating stress-enduring (Muse) cells are a unique subpopulation of mesenchymal stem cells (MSCs) that can tolerate stress and differentiate into multiple cell types. However, the effects of Muse cell-derived exosomes (Muse-Exos) on melanocyte injury are not well understood. In this study, we investigated whether Muse-Exos could protect human epidermal melanocytes (HEMs) from hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced oxidative stress injury in vitro. Muse cells were isolated from MSCs via magnetic-activated cell sorting (MACS), and the exosomes were collected by ultracentrifugation. The exosomes were characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and Western blotting. HEMs were pretreated with either MSC-Exos or Muse-Exos at concentrations of 5, 10–20&#xa0;µg/mL for 48&#xa0;h, after which they were exposed to H<sub>2</sub>O<sub>2</sub> to induce oxidative stress. Functional assays were conducted to assess cell viability, apoptosis, reactive oxygen species (ROS) accumulation and autophagy-related markers (LC3B-II, Beclin-1 and p62). Both MSC-Exos and Muse-Exos reduced H<sub>2</sub>O<sub>2</sub>-induced cytotoxicity and promoted cell survival. Muse-Exos were found to have relatively stronger effects in reducing ROS levels, inhibiting apoptosis, and enhancing autophagic activity. These results suggest that Muse-Exos may protect melanocytes against oxidative stress in vitro and could inform new approaches to treating vitiligo using exosomes.</p>

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Exosomes from multilineage differentiating stress-enduring (Muse) cells attenuate H2O2-induced melanocyte injury in vitro

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月20日

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  • Mingmei Yang,
  • Huiying Wang,
  • Zeyu Huang,
  • Ruzhi Zhang

摘要

Mesenchymal stem cell-derived exosomes (MSC-Exos) have been shown to protect melanocytes from oxidative injury, suggesting their potential contribution to therapeutic strategies for vitiligo. Multilineage-differentiating stress-enduring (Muse) cells are a unique subpopulation of mesenchymal stem cells (MSCs) that can tolerate stress and differentiate into multiple cell types. However, the effects of Muse cell-derived exosomes (Muse-Exos) on melanocyte injury are not well understood. In this study, we investigated whether Muse-Exos could protect human epidermal melanocytes (HEMs) from hydrogen peroxide (H2O2)-induced oxidative stress injury in vitro. Muse cells were isolated from MSCs via magnetic-activated cell sorting (MACS), and the exosomes were collected by ultracentrifugation. The exosomes were characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and Western blotting. HEMs were pretreated with either MSC-Exos or Muse-Exos at concentrations of 5, 10–20 µg/mL for 48 h, after which they were exposed to H2O2 to induce oxidative stress. Functional assays were conducted to assess cell viability, apoptosis, reactive oxygen species (ROS) accumulation and autophagy-related markers (LC3B-II, Beclin-1 and p62). Both MSC-Exos and Muse-Exos reduced H2O2-induced cytotoxicity and promoted cell survival. Muse-Exos were found to have relatively stronger effects in reducing ROS levels, inhibiting apoptosis, and enhancing autophagic activity. These results suggest that Muse-Exos may protect melanocytes against oxidative stress in vitro and could inform new approaches to treating vitiligo using exosomes.