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<p>Androgenetic alopecia (AGA) stems from the reaction of hair follicles to circulating testosterone and its derivatives, resulting in progressive hair loss. It is the most common cause of hair loss in both men and women. Human amniotic mesenchymal stem cells (hAMSCs) have multilineage differentiation, immunosuppressive, and anti-inflammatory potential which makes them suitable for treating AGA. This study aimed to explore the effect and mechanism of hAMSCs conditional medium (hAMSC-CM) and lysate (hAMSC-Lys) on AGA. hAMSCs were isolated from the amniotic membrane and characterized by flow cytometry, immunofluorescence, and adipogenic and osteogenic differentiation. We assessed the effects of hAMSC-Lys and hAMSC-CM on the normal hair growth cycle and AGA mice, respectively. We then explored its underlying mechanisms by assessing angiogenesis, proliferation of dermal papilla cells (DPCs), and inflammation levels in the skin tissues of normal mice and AGA mice treated with water, minoxidil, hAMSC-CM, or hAMSC-Lys. Antibody array assay and mass spectrometry were respectively used to identify the cytokines in hAMSC-CM and the proteins in hAMSC-Lys that may promote hair regeneration in AGA mice. hAMSCs expressed various markers of embryonic stem cells and MSCs and have the potential to differentiate into adipocytes and osteocytes. hAMSC-CM and hAMSC-Lys microneedle treatment significantly promoted the hair growth cycle of normal mice and hair regeneration in AGA mice. Furthermore, we demonstrated that hAMSC-CM and hAMSC-Lys promoted hair regeneration in AGA mice through enhancing angiogenesis, proliferation of DPCs, and suppressing inflammation. Antibody array assay and mass spectrometry showed that hAMSC-CM and hAMSC-Lys contain a panel of cytokines or proteins that can promote hair regeneration in AGA mice. Our results demonstrated that hAMSC-CM and hAMSC-Lys significantly prevented hair regression induced by androgen stimulation by promoting angiogenesis, increasing the proliferation of DPCs, and suppressing inflammation, suggesting that hAMSC-CM and hAMSC-Lys may provide an alternative therapeutic approach for the treatment of AGA.</p>

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Human amniotic mesenchymal stem cell conditioned medium and its lysate promote hair follicle regeneration in androgenetic alopecia mice

  • 期刊论文

  • 发表日期：

    2025年10月17日

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  • Wei-Ran Jin,
  • Shu-Yang He,
  • Bing-Qian Ji,
  • Wei-Fen Cheng,
  • Nan-Yang Zhang,
  • Ling-Fei Yan,
  • Jing-Yuan Li,
  • Xiang-Cheng Zhang

摘要

Androgenetic alopecia (AGA) stems from the reaction of hair follicles to circulating testosterone and its derivatives, resulting in progressive hair loss. It is the most common cause of hair loss in both men and women. Human amniotic mesenchymal stem cells (hAMSCs) have multilineage differentiation, immunosuppressive, and anti-inflammatory potential which makes them suitable for treating AGA. This study aimed to explore the effect and mechanism of hAMSCs conditional medium (hAMSC-CM) and lysate (hAMSC-Lys) on AGA. hAMSCs were isolated from the amniotic membrane and characterized by flow cytometry, immunofluorescence, and adipogenic and osteogenic differentiation. We assessed the effects of hAMSC-Lys and hAMSC-CM on the normal hair growth cycle and AGA mice, respectively. We then explored its underlying mechanisms by assessing angiogenesis, proliferation of dermal papilla cells (DPCs), and inflammation levels in the skin tissues of normal mice and AGA mice treated with water, minoxidil, hAMSC-CM, or hAMSC-Lys. Antibody array assay and mass spectrometry were respectively used to identify the cytokines in hAMSC-CM and the proteins in hAMSC-Lys that may promote hair regeneration in AGA mice. hAMSCs expressed various markers of embryonic stem cells and MSCs and have the potential to differentiate into adipocytes and osteocytes. hAMSC-CM and hAMSC-Lys microneedle treatment significantly promoted the hair growth cycle of normal mice and hair regeneration in AGA mice. Furthermore, we demonstrated that hAMSC-CM and hAMSC-Lys promoted hair regeneration in AGA mice through enhancing angiogenesis, proliferation of DPCs, and suppressing inflammation. Antibody array assay and mass spectrometry showed that hAMSC-CM and hAMSC-Lys contain a panel of cytokines or proteins that can promote hair regeneration in AGA mice. Our results demonstrated that hAMSC-CM and hAMSC-Lys significantly prevented hair regression induced by androgen stimulation by promoting angiogenesis, increasing the proliferation of DPCs, and suppressing inflammation, suggesting that hAMSC-CM and hAMSC-Lys may provide an alternative therapeutic approach for the treatment of AGA.