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<p>The purpose of this study was to investigate whether subcutaneous injection of conditioned medium (CM) from photobiomodulation (PBM) irradiated adipose-derived stromal cells (ASCs) could stimulate hair growth in mice. Human ASCs were plated on cell culture dishes at a concentration of 2 × 10<sup>4</sup> cells/cm<sup>2</sup> and allowed to adhere at 37&#xa0;°C. Light emitting diode (660&#xa0;nm, 50 mW/cm<sup>2</sup>) was applied for 10&#xa0;min daily from day 1 to day 10. CM fractions were collected from PBM-irradiated ASCs to yield (PBM-CM). They were centrifuged at 13,000&#xa0;g for 10&#xa0;min at 4°C and stored prior to use for ELISA, protein assay, or in vivo assays. Forty-one growth factors in PBM-CM from cells cultured under PBM or non-PBM conditions were analyzed. Survival, differentiation and secretion of vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), and hepatocyte growth factor (HGF) of PBM-CM were evaluated by western blot. Secretion of insulin-like growth (IGF), platelet-derived growth factor (PDGF), and insulin-like growth factor binding protein (IGFBP) were significantly increased by PBM. PBM-CM enhanced hair growth in a hair loss model compared to non-irradiated ASC-CM or Phosphate-buffered saline (PBS). PBM is an effective biostimulator of ASC-CM in hair regrowth by enhancing secretion of growth factors. Thus, PBM-CM has the potential to stimulate hair growth and treat alopecia.</p>

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Subcutaneous injection of conditioned medium from photobiomodulation-treated adipose-derived stromal cells promotes hair growth and angiogenesis in mice

  • 期刊论文

  • 发表日期：

    2025年6月14日

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  • In-Su Park

摘要

The purpose of this study was to investigate whether subcutaneous injection of conditioned medium (CM) from photobiomodulation (PBM) irradiated adipose-derived stromal cells (ASCs) could stimulate hair growth in mice. Human ASCs were plated on cell culture dishes at a concentration of 2 × 104 cells/cm2 and allowed to adhere at 37 °C. Light emitting diode (660 nm, 50 mW/cm2) was applied for 10 min daily from day 1 to day 10. CM fractions were collected from PBM-irradiated ASCs to yield (PBM-CM). They were centrifuged at 13,000 g for 10 min at 4°C and stored prior to use for ELISA, protein assay, or in vivo assays. Forty-one growth factors in PBM-CM from cells cultured under PBM or non-PBM conditions were analyzed. Survival, differentiation and secretion of vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), and hepatocyte growth factor (HGF) of PBM-CM were evaluated by western blot. Secretion of insulin-like growth (IGF), platelet-derived growth factor (PDGF), and insulin-like growth factor binding protein (IGFBP) were significantly increased by PBM. PBM-CM enhanced hair growth in a hair loss model compared to non-irradiated ASC-CM or Phosphate-buffered saline (PBS). PBM is an effective biostimulator of ASC-CM in hair regrowth by enhancing secretion of growth factors. Thus, PBM-CM has the potential to stimulate hair growth and treat alopecia.