Scholarlink

Background <p>Poorly healing wounds represent the primary health-related burden for hereditary recessive dystrophic epidermolysis bullosa (RDEB) patients. Contribution of wound-associated soluble constituents to wound progression remains not well defined.</p> Objective <p>To conduct cross-sectional analysis of cytokine, chemokine, and growth factor in exudates from RDEB wounds and define changes associated with wound progression.</p> Methods <p>Concentrations of selected cytokines, chemokines, and growth factors were evaluated by multiplex ELISA in eight blister fluids and 66 exudates from early, established, and chronic RDEB and five chronic venous ulcers (VU). A cross-sectional analysis was performed.</p> Results <p>Our data demonstrated that proinflammatory CXCL8 and IL-1β tend to accumulate in established RDEB lesions. The levels of several interleukins including IL-17, IL-18, and IL-10 were significantly higher in RDEB chronic wounds than in VU. Contrary to VU and other chronic wounds, high levels of VEGF, G-CSF, and HGF growth factors were detected in RDEB established and chronic skin lesions.</p> Conclusion <p>Although this study is limited to cross-sectional analysis of wound exudates, detected high levels of specific pro-inflammatory, neutrophil-recruiting, and pro-angiogenic and pro-proliferative factors, such as IL-1β, CXCL8, VEGF, G-CSF, and HGF define RDEB wounds and offer potential pharmacological targets to improve wound healing in the patients.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Cross-sectional analysis of wound-associated soluble factors in early, established, and chronic wounds of recessive dystrophic epidermolysis bullosa patients

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年6月4日

查看全文
  • Vitali Alexeev,
  • Leonie Huitema,
  • Taylor Phillips,
  • Paras Patel,
  • Mauricio Salas Garza,
  • Franziska Ringpfeil,
  • Julio Cesar Salas-Alanis,
  • Olga Igoucheva

摘要

Background

Poorly healing wounds represent the primary health-related burden for hereditary recessive dystrophic epidermolysis bullosa (RDEB) patients. Contribution of wound-associated soluble constituents to wound progression remains not well defined.

Objective

To conduct cross-sectional analysis of cytokine, chemokine, and growth factor in exudates from RDEB wounds and define changes associated with wound progression.

Methods

Concentrations of selected cytokines, chemokines, and growth factors were evaluated by multiplex ELISA in eight blister fluids and 66 exudates from early, established, and chronic RDEB and five chronic venous ulcers (VU). A cross-sectional analysis was performed.

Results

Our data demonstrated that proinflammatory CXCL8 and IL-1β tend to accumulate in established RDEB lesions. The levels of several interleukins including IL-17, IL-18, and IL-10 were significantly higher in RDEB chronic wounds than in VU. Contrary to VU and other chronic wounds, high levels of VEGF, G-CSF, and HGF growth factors were detected in RDEB established and chronic skin lesions.

Conclusion

Although this study is limited to cross-sectional analysis of wound exudates, detected high levels of specific pro-inflammatory, neutrophil-recruiting, and pro-angiogenic and pro-proliferative factors, such as IL-1β, CXCL8, VEGF, G-CSF, and HGF define RDEB wounds and offer potential pharmacological targets to improve wound healing in the patients.