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<p>The <i>Nymphaea hybrid</i> stems, as industrially harvested waste parts of <i>Nymphaea hybrid</i>, have received limited attention, particularly as a source of bioactive polysaccharides. Enzymatic-assisted Flash Extraction (EAFE) for effective polysaccharide extraction from <i>Nymphaea hybrid</i> stems were optimized using response surface techniques, and the optimal parameters were established as follows: A-Enzymatic hydrolysis temperature(50 ℃), B-pH value(5), and C-Flash extraction time(70&#xa0;s), yielding 216 ± 1.24&#xa0;mg/g of crude <i>Nymphaea hybrid</i> stems polysaccharide (NHSP). Two fractions from NHSP, NHSP-1 (10.3&#xa0;kDa) and NHSP-2 (17.7&#xa0;kDa), were isolated and refined using DEAE-52 and Sephadex G-100. NHSP-1 is a glucose-based polymer, while NHSP-2 is a heteropolysaccharide including nine monosaccharides. Differences were observed in their surface morphology, with NHSP-1 presenting a lamellar spherical architecture and NHSP-2 showing a loose, web-like structure. Bioactivity assays revealed that NHSPs possess potential antioxidant, anti-inflammatory, and moisture retention properties. However, the distinct structural features of NHSP-1 and NHSP-2 endow them with different efficacy properties. These findings offer theoretical support for the optimization of extraction efficiency and refined utilization of NHSP, offering potential for creating targeted functional ingredients in nutraceutical and cosmetic applications.</p>

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Optimization of polysaccharides extraction from Nymphaea hybrid stems by enzymatic-assisted flash extraction method and evaluation of its antioxidant, anti-inflammatory, and moisture-retention activities

  • 期刊论文

  • 发表日期：

    2025年11月28日

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  • Huimin Liu,
  • Xinyi Ma,
  • Mingyan Chen,
  • Xiang Ma,
  • Hao Zeng,
  • Yun Zhang,
  • Xiaoyi Wang,
  • Wei Wang

摘要

The Nymphaea hybrid stems, as industrially harvested waste parts of Nymphaea hybrid, have received limited attention, particularly as a source of bioactive polysaccharides. Enzymatic-assisted Flash Extraction (EAFE) for effective polysaccharide extraction from Nymphaea hybrid stems were optimized using response surface techniques, and the optimal parameters were established as follows: A-Enzymatic hydrolysis temperature(50 ℃), B-pH value(5), and C-Flash extraction time(70 s), yielding 216 ± 1.24 mg/g of crude Nymphaea hybrid stems polysaccharide (NHSP). Two fractions from NHSP, NHSP-1 (10.3 kDa) and NHSP-2 (17.7 kDa), were isolated and refined using DEAE-52 and Sephadex G-100. NHSP-1 is a glucose-based polymer, while NHSP-2 is a heteropolysaccharide including nine monosaccharides. Differences were observed in their surface morphology, with NHSP-1 presenting a lamellar spherical architecture and NHSP-2 showing a loose, web-like structure. Bioactivity assays revealed that NHSPs possess potential antioxidant, anti-inflammatory, and moisture retention properties. However, the distinct structural features of NHSP-1 and NHSP-2 endow them with different efficacy properties. These findings offer theoretical support for the optimization of extraction efficiency and refined utilization of NHSP, offering potential for creating targeted functional ingredients in nutraceutical and cosmetic applications.