Scholarlink

<p>The performance of chromatographic media affects the efficiency of purification. In this study, based on a T-type microfluidic droplet generation system, agarose microspheres with large pore size and high load were prepared by adjusting the viscosity of a 4% agarose solution to 70&#xa0;mPa·s at high temperature. Optical microscopy revealed that the self-made agarose microspheres had a good spherical structure with an average particle size of 82.81&#xa0;μm and a CV value of 0.0576. Subsequently, the stability and mechanical strength of the self-made agarose microspheres were improved by cross-linking and activation with epichlorohydrin. After the cross-linking and activation, the agarose microspheres were grafted with Protein A to make it have specific adsorption capacity for IgG. The adsorption capacity of the self-made agarose microspheres grafted with Protein A for IgG was 87&#xa0;mg/g. In the complex serum environment, the agarose microspheres grafted with Protein A still maintained good IgG adsorption capacity and selectivity, demonstrating their potential in practical applications. This study demonstrates the great potential of microfluidic technology in the design and preparation of bio-separation media. By precisely controlling the particle size and pore size of the microspheres, the purification efficiency and selectivity can be significantly improved, providing a new solution for the efficient purification of bio-products.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Preparation of agarose microspheres with large pore size and high load and purification of antibody in serum based on microfluidic technology

  • 期刊论文

  • 发表日期：

    2025年1月20日

查看全文
  • Guanglei Chang,
  • Miaomiao Yang,
  • Zhaobin Xu,
  • Xinling Wang,
  • Wenyao Li,
  • Meixia He,
  • Jintao Zhang,
  • Yuanzhi Xu,
  • Lulu Wang,
  • Liguo Zhang

摘要

The performance of chromatographic media affects the efficiency of purification. In this study, based on a T-type microfluidic droplet generation system, agarose microspheres with large pore size and high load were prepared by adjusting the viscosity of a 4% agarose solution to 70 mPa·s at high temperature. Optical microscopy revealed that the self-made agarose microspheres had a good spherical structure with an average particle size of 82.81 μm and a CV value of 0.0576. Subsequently, the stability and mechanical strength of the self-made agarose microspheres were improved by cross-linking and activation with epichlorohydrin. After the cross-linking and activation, the agarose microspheres were grafted with Protein A to make it have specific adsorption capacity for IgG. The adsorption capacity of the self-made agarose microspheres grafted with Protein A for IgG was 87 mg/g. In the complex serum environment, the agarose microspheres grafted with Protein A still maintained good IgG adsorption capacity and selectivity, demonstrating their potential in practical applications. This study demonstrates the great potential of microfluidic technology in the design and preparation of bio-separation media. By precisely controlling the particle size and pore size of the microspheres, the purification efficiency and selectivity can be significantly improved, providing a new solution for the efficient purification of bio-products.

Graphical abstract