Purpose Folate, also called vitamin B9, plays a key role in various cellular processes that support T cell proliferation and function, which in turn regulate adaptive immune responses. Nonetheless, the associations between serum folate concentrations and specific T cell subsets remain underexplored. Methods Data of the population-based MEIA study were analyzed including 594 participants aged 19–75 years in Augsburg study region, Germany. Serum folate concentrations were measured (right-censored at 20 ng/mL) and immunophenotyping of T cells via flow cytometry was performed in fresh blood samples. Associations between folate concentrations and T cell subsets were analyzed using a multivariable two-stage regression model to account for censoring. Results Positive associations were noted with Effector Memory CD8 + T cells (<InlineEquation ID="IEq1"> <EquationSource Format="TEX">\(\:\beta\:\)</EquationSource> </InlineEquation>= 0.39; 95% CI [0.09;0.70]), CD8+ CD27- CD28+ T cells (<InlineEquation ID="IEq2"> <EquationSource Format="TEX">\(\:\beta\:\)</EquationSource> </InlineEquation>= 1.02; 95% CI [1.00; 1.03]), and CD8+ CD27+ CD28- T cells (<InlineEquation ID="IEq3"> <EquationSource Format="TEX">\(\:\beta\:\)</EquationSource> </InlineEquation>= 1.01; 95% CI [1.00; 1.03]), negative associations were observed with Central Memory CD8+ T cells (<InlineEquation ID="IEq4"> <EquationSource Format="TEX">\(\:\beta\:\)</EquationSource> </InlineEquation>= − 0.29; 95% CI [− 0.47; − 0.10]) and naïve CD8+ T cells (<InlineEquation ID="IEq5"> <EquationSource Format="TEX">\(\:\beta\:\)</EquationSource> </InlineEquation>= − 0.33; 95% CI [− 0.63; − 0.04]). Although these associations did not retain statistical significance after adjustment for multiple testing, they were fully supported by sensitivity analyses. Conclusion This study provides preliminary evidence linking serum folate to specific T cell subsets, particularly within CD8 + populations. While findings are suggestive, they emphasize the potential importance of adequate folate levels for immune health. Further longitudinal and interventional studies are needed to confirm these associations and explore the role of folate in immune function.

Association between serum folate and T cell subsets in a population-based study

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2026年1月16日

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Laura Vanessa Stoß,
Christa Meisinger,
Florian Rohm,
Jakob Linseisen,
Dennis Freuer

摘要

Purpose

Folate, also called vitamin B9, plays a key role in various cellular processes that support T cell proliferation and function, which in turn regulate adaptive immune responses. Nonetheless, the associations between serum folate concentrations and specific T cell subsets remain underexplored.

Methods

Data of the population-based MEIA study were analyzed including 594 participants aged 19–75 years in Augsburg study region, Germany. Serum folate concentrations were measured (right-censored at 20 ng/mL) and immunophenotyping of T cells via flow cytometry was performed in fresh blood samples. Associations between folate concentrations and T cell subsets were analyzed using a multivariable two-stage regression model to account for censoring.

Results

Positive associations were noted with Effector Memory CD8 + T cells ( \(\:\beta\:\) = 0.39; 95% CI [0.09;0.70]), CD8+ CD27- CD28+ T cells ( \(\:\beta\:\) = 1.02; 95% CI [1.00; 1.03]), and CD8+ CD27+ CD28- T cells ( \(\:\beta\:\) = 1.01; 95% CI [1.00; 1.03]), negative associations were observed with Central Memory CD8+ T cells ( \(\:\beta\:\) = − 0.29; 95% CI [− 0.47; − 0.10]) and naïve CD8+ T cells ( \(\:\beta\:\) = − 0.33; 95% CI [− 0.63; − 0.04]). Although these associations did not retain statistical significance after adjustment for multiple testing, they were fully supported by sensitivity analyses.

Conclusion

This study provides preliminary evidence linking serum folate to specific T cell subsets, particularly within CD8 + populations. While findings are suggestive, they emphasize the potential importance of adequate folate levels for immune health. Further longitudinal and interventional studies are needed to confirm these associations and explore the role of folate in immune function.