Scholarlink

<p>Didecyl dimethyl ammonium chloride (DDAC) is a widely used quaternary ammonium disinfectant whose persistence in soil poses a potential risk to microbial ecosystems. This study investigated the effect of DDAC on soil microbial communities at a realistic field concentration (0.1&#xa0;µg/g) and a mechanism-probing concentration (10.0&#xa0;µg/g) over 120 days. Results revealed the dual effects: (1) DDAC significantly enhanced the genetic potential for nitrogen cycling, increasing the abundance of key functional genes (<i>AOA-amoA</i>, <i>AOB-amoA</i>, <i>nirK</i>, <i>nirS</i>, <i>nosZ</i>) by up to 2.66-fold; (2) In contrast, it suppressed microbial carbon metabolism, evidenced by reduced β-glucosidase (β-GC) activity under 0.1&#xa0;µg/g DDAC during days 0–7, consistently inhibited soil dehydrogenase (DHA) activity at 10.0&#xa0;µg/g, accompanied by an overall decline in the carbon substrate utilization potential of the culturable microbial fraction. DDAC exposure also reduced microbial diversity, restructured community composition, and induced a loosening of fungal co-occurrence networks. Notably, DDAC amplified antibiotic resistance dissemination. The low concentration (0.1&#xa0;µg/g) DDAC promoted significant correlations between the transposase gene <i>tnpA-04</i> and aminoglycoside and multidrug antibiotic resistance genes. The high DDAC concentration (10.0&#xa0;µg/g) induced the persistent emergence of the disinfectant resistance gene <i>qacH-01</i> and enhanced co-occurrence between multidrug resistance genes and mobile genetic elements. The lack of identifiable bacterial hosts for key genetic elements in co-occurrence networks implies that horizontal gene transfer likely contributes to resistance dissemination. These findings indicate that under the soil conditions selected for this study, DDAC may affect soil biogeochemical cycles and to some extent promote the spread of antibiotic resistance, providing important reference for its environmental risk assessment.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The soil treatment trade-off of didecyl dimethyl ammonium chloride: stimulated nitrogen cycling against a backdrop of impaired carbon metabolism and expanding antibiotic resistance

  • 期刊论文

  • 发表日期：

    2026年4月16日

查看全文
  • Lilan Zhang,
  • Wenwen Li,
  • Qianyi Jin,
  • Kejin Chen

摘要

Didecyl dimethyl ammonium chloride (DDAC) is a widely used quaternary ammonium disinfectant whose persistence in soil poses a potential risk to microbial ecosystems. This study investigated the effect of DDAC on soil microbial communities at a realistic field concentration (0.1 µg/g) and a mechanism-probing concentration (10.0 µg/g) over 120 days. Results revealed the dual effects: (1) DDAC significantly enhanced the genetic potential for nitrogen cycling, increasing the abundance of key functional genes (AOA-amoA, AOB-amoA, nirK, nirS, nosZ) by up to 2.66-fold; (2) In contrast, it suppressed microbial carbon metabolism, evidenced by reduced β-glucosidase (β-GC) activity under 0.1 µg/g DDAC during days 0–7, consistently inhibited soil dehydrogenase (DHA) activity at 10.0 µg/g, accompanied by an overall decline in the carbon substrate utilization potential of the culturable microbial fraction. DDAC exposure also reduced microbial diversity, restructured community composition, and induced a loosening of fungal co-occurrence networks. Notably, DDAC amplified antibiotic resistance dissemination. The low concentration (0.1 µg/g) DDAC promoted significant correlations between the transposase gene tnpA-04 and aminoglycoside and multidrug antibiotic resistance genes. The high DDAC concentration (10.0 µg/g) induced the persistent emergence of the disinfectant resistance gene qacH-01 and enhanced co-occurrence between multidrug resistance genes and mobile genetic elements. The lack of identifiable bacterial hosts for key genetic elements in co-occurrence networks implies that horizontal gene transfer likely contributes to resistance dissemination. These findings indicate that under the soil conditions selected for this study, DDAC may affect soil biogeochemical cycles and to some extent promote the spread of antibiotic resistance, providing important reference for its environmental risk assessment.