Scholarlink

<p><i>Phytophthora nicotianae</i>, <i>Meloidogyne incognita</i>, and <i>Ralstonia solanacearum</i> are the causal agents of black shank, root-knot nematode disease, and bacterial wilt in tobacco (<i>Nicotiana tabacum</i>), respectively. These pathogens not only cause severe growth inhibition and yield losses but also alter the microbial composition of root-associated compartments. Nevertheless, a comprehensive understanding of their impact on the composition and function of these microbial communities remains limited. In this study, we investigated variations in the composition and function of microbial communities in the endosphere, rhizosphere, and root-adjacent soil of both susceptible and tolerant tobacco varieties inoculated with the three pathogens, using 16&#xa0;S rRNA amplicon and metagenomic sequencing. Additionally, soil-derived bacterial isolates were screened and characterized for their potential biocontrol activity against the pathogens. The composition and diversity of root-associated microbial communities differed significantly under different pathogen inoculations. Proteobacteria, Actinobacteriota, Cyanobacteria and Acidobacteriota were identified as the dominant phyla across treatments. Notably, opposing trends in the relative abundance of <i>Cyanobacteria</i> and <i>Acidobacteriota</i> were observed in response to <i>P. nicotianae</i> infection (black shank), whereas similar trends were demonstrated following <i>M. incognita</i> (root-knot nematodes) and R. <i>solanacearum</i> infection (bacterial wilt). Furthermore, the composition of endospheric microbial communities shifted markedly with plant developmental stages, suggesting dynamic interactions between plant growth and microbial colonization. These findings offer new insights into the regulatory mechanisms by which pathogens modulate plant microbiomes and highlight potential microbial indicators that may inform breeding strategies for disease-resistant tobacco varieties.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Root-associated microbiome dynamics reveal ecological responses to soil-borne pathogens in susceptible and tolerant tobacco varieties

  • 期刊论文

  • 发表日期：

    2025年10月9日

查看全文
  • Yi Cao,
  • Ning Lu,
  • Dongmei Yang,
  • Xingjiang Chen,
  • Xianbin Yang

摘要

Phytophthora nicotianae, Meloidogyne incognita, and Ralstonia solanacearum are the causal agents of black shank, root-knot nematode disease, and bacterial wilt in tobacco (Nicotiana tabacum), respectively. These pathogens not only cause severe growth inhibition and yield losses but also alter the microbial composition of root-associated compartments. Nevertheless, a comprehensive understanding of their impact on the composition and function of these microbial communities remains limited. In this study, we investigated variations in the composition and function of microbial communities in the endosphere, rhizosphere, and root-adjacent soil of both susceptible and tolerant tobacco varieties inoculated with the three pathogens, using 16 S rRNA amplicon and metagenomic sequencing. Additionally, soil-derived bacterial isolates were screened and characterized for their potential biocontrol activity against the pathogens. The composition and diversity of root-associated microbial communities differed significantly under different pathogen inoculations. Proteobacteria, Actinobacteriota, Cyanobacteria and Acidobacteriota were identified as the dominant phyla across treatments. Notably, opposing trends in the relative abundance of Cyanobacteria and Acidobacteriota were observed in response to P. nicotianae infection (black shank), whereas similar trends were demonstrated following M. incognita (root-knot nematodes) and R. solanacearum infection (bacterial wilt). Furthermore, the composition of endospheric microbial communities shifted markedly with plant developmental stages, suggesting dynamic interactions between plant growth and microbial colonization. These findings offer new insights into the regulatory mechanisms by which pathogens modulate plant microbiomes and highlight potential microbial indicators that may inform breeding strategies for disease-resistant tobacco varieties.