For a graph G with vertex assignment \(c:V(G)\rightarrow \mathbb {Z}^+\) , we define \(\sum _{v\in V(H)}c(v)\) for a connected subgraph H of G as a connected subgraph sum of G. We study the set S(G, c) of connected subgraph sums and, in particular, resolve a problem posed by O.-H. S. Lo in a strong form. We show that for each n-vertex graph G, there is a vertex assignment \(c:V(G)\rightarrow \{1,\dots ,12n^2\}\) such that for every n-vertex graph \(G'\not \cong G\) and vertex assignment \(c'\) for \(G'\) , the corresponding collections of connected subgraph sums are different (i.e., \(S(G,c)\ne S(G',c')\) ). We also provide some remarks on vertex assignments of a graph G for which all connected subgraph sums are different.