Explicit illumination correction for error reduction in two-dye LIF thermometry
摘要
We propose a method to perform accurate temperature measurements using laser-induced fluorescence (LIF). We use a sCMOS color camera and a two-dye solution consisting of RuPhen and fluorescein, excited at 450 nm. By varying the relative concentration of the dyes, we can tune the temperature sensitivity of the color channels. This enables a robust laser power correction, reducing the effects of experimental noise compared to the convectional ratiometric approach. Furthermore, the overall temperature sensitivity is only slightly lower compared to that of the temperature-sensitive dye, which is not the case if a ratiometric analysis is performed. We demonstrate the capabilities of our method using a benchtop setup with precisely controlled temperatures. Our error analysis shows that an accuracy better than 0.5