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<p>The mitogen-activated protein kinase (MAPK) plays important roles in stress signaling and development. Nevertheless, the functions of <i>MAPK</i> genes in moso bamboo (<i>Phyllostachys edulis</i>) remain largely uncharacterized. Here, a total of 29 moso bamboo <i>MAPK</i> genes were unevenly distributed across 14 chromosomes were classified into four subfamilies. An in-depth investigation of conserved motifs, gene structure, <i>cis</i>-acting elements, chromosomal localization, and expression patterns of <i>MAPK</i> genes in moso bamboo was carried out. WGD or Segmental duplication was the main driving force for the expansion of the PeMAPK family. All duplicated gene pairs have undergone purifying selection. Expression analysis indicated variation in expression levels of <i>PeMAPK</i>s across different tissues, and most PeMAPKs responded to at least one stress. Notably, <i>PeMAPK8</i> showed marked up-regulation under salt, drought, and cold stresses, and was thus selected for further investigation. Subcellular localization showed that PeMAPK8 was localized in the nucleus. Overexpression of <i>PeMAPK8</i> in <i>Arabidopsis thaliana</i> significantly enhanced salt tolerance, evidenced by increased germination rate and reduced leaf damage under salt treatment. This study enriched the knowledge of MAPKs in moso bamboo and provided genetic resources for its stress-resistant breeding.</p>

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Characterization of MAPK Gene Family in Moso Bamboo (Phyllostachys edulis) and Functional Analysis of PeMAPK8 Under Salt Stress

  • 期刊论文

  • 发表日期：

    2026年8月4日

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  • Tiantian Lu,
  • Xueyi Chen,
  • Yujie Mao,
  • Feiyi Huang

摘要

The mitogen-activated protein kinase (MAPK) plays important roles in stress signaling and development. Nevertheless, the functions of MAPK genes in moso bamboo (Phyllostachys edulis) remain largely uncharacterized. Here, a total of 29 moso bamboo MAPK genes were unevenly distributed across 14 chromosomes were classified into four subfamilies. An in-depth investigation of conserved motifs, gene structure, cis-acting elements, chromosomal localization, and expression patterns of MAPK genes in moso bamboo was carried out. WGD or Segmental duplication was the main driving force for the expansion of the PeMAPK family. All duplicated gene pairs have undergone purifying selection. Expression analysis indicated variation in expression levels of PeMAPKs across different tissues, and most PeMAPKs responded to at least one stress. Notably, PeMAPK8 showed marked up-regulation under salt, drought, and cold stresses, and was thus selected for further investigation. Subcellular localization showed that PeMAPK8 was localized in the nucleus. Overexpression of PeMAPK8 in Arabidopsis thaliana significantly enhanced salt tolerance, evidenced by increased germination rate and reduced leaf damage under salt treatment. This study enriched the knowledge of MAPKs in moso bamboo and provided genetic resources for its stress-resistant breeding.