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<p>Tomatoes are often susceptible to damage during cold storage. In the present study, we researched how <i>SlBRI1</i> regulates the cold storage tolerance of tomato fruits at both the physiological and transcriptomic levels. We found that low temperatures induced the expression of the brassinosteroids (BRs) receptor BRASSINOSTEROID INSENSITIVE 1 (BRI1), which peaked at 16&#xa0;h and then declined. Fruit surface lesions on plants with overexpression of <i>SlBRI1</i> (<i>SlBRI1OE</i>) were less severe than in WT fruits during cold storage. In the fruits of <i>SlBRI1OE</i> plants, relative weight loss rate, ion leakage, malondialdehyde level and, O<sub>2</sub><sup>−</sup> and H<sub>2</sub>O<sub>2</sub> contents were all significantly lower in fruits from WT plants. In contrast, proline and soluble protein contents, superoxide dismutase (SOD) and peroxidase (POD) activity were significantly higher in <i>SlBRI1OE</i> fruits than in WT fruit during cold storage. Furthermore, the activity of LOX and PLD were significantly lower in <i>SlBRI1OE</i> fruits than in WT fruits. The expression levels of <i>SlICE1</i>, <i>SlCBF1</i>, <i>SlCBF3</i> and <i>SlDRCi7</i> were higher in <i>SlBRI1OE</i> fruits than in WT fruits. RNA sequence analysis further showed that the expression levels of one photosystem I and eight photosystem II protein genes, seven heat shock proteins, two key ABA biosynthesis enzymes, and one ABA stress-inducible protein gene in <i>SlBRI1OE</i> fruit was higher than in WT fruit during cold storage. Our results indicate that <i>SlBRI1</i> plays a crucial role in cold-storage tolerance in tomato fruits. Therefore, our study provides valuable information for future breeding to improve the expression of <i>SlBRI1</i> and enhance cold tolerance of tomato fruits during cold storage.</p>

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Overexpression of Brassinosteroids Receptor SlBRI1 Improves Chilling Stress Tolerance of Tomato Fruits During Cold Storage

  • 期刊论文

  • 发表日期：

    2026年7月28日

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  • Wenwen Yang,
  • Juanfeng Liu,
  • Jiajia Li,
  • Can Xie,
  • Shuming Nie,
  • Dan Wang

摘要

Tomatoes are often susceptible to damage during cold storage. In the present study, we researched how SlBRI1 regulates the cold storage tolerance of tomato fruits at both the physiological and transcriptomic levels. We found that low temperatures induced the expression of the brassinosteroids (BRs) receptor BRASSINOSTEROID INSENSITIVE 1 (BRI1), which peaked at 16 h and then declined. Fruit surface lesions on plants with overexpression of SlBRI1 (SlBRI1OE) were less severe than in WT fruits during cold storage. In the fruits of SlBRI1OE plants, relative weight loss rate, ion leakage, malondialdehyde level and, O2− and H2O2 contents were all significantly lower in fruits from WT plants. In contrast, proline and soluble protein contents, superoxide dismutase (SOD) and peroxidase (POD) activity were significantly higher in SlBRI1OE fruits than in WT fruit during cold storage. Furthermore, the activity of LOX and PLD were significantly lower in SlBRI1OE fruits than in WT fruits. The expression levels of SlICE1, SlCBF1, SlCBF3 and SlDRCi7 were higher in SlBRI1OE fruits than in WT fruits. RNA sequence analysis further showed that the expression levels of one photosystem I and eight photosystem II protein genes, seven heat shock proteins, two key ABA biosynthesis enzymes, and one ABA stress-inducible protein gene in SlBRI1OE fruit was higher than in WT fruit during cold storage. Our results indicate that SlBRI1 plays a crucial role in cold-storage tolerance in tomato fruits. Therefore, our study provides valuable information for future breeding to improve the expression of SlBRI1 and enhance cold tolerance of tomato fruits during cold storage.