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<p><i>Rosa rugosa</i> Thunb. (<i>R. rugosa</i>) possesses considerable medicinal value and has attracted global interest. Given that bioactive metabolites may vary during floral development, we employed transcriptomic and metabolomic approaches to elucidate their regulatory mechanisms. A total of 205 differentially accumulated metabolites and 6,303 differentially expressed genes were identified. Integrated transcriptome and metabolome analysis revealed significant enrichment in five key metabolic pathways: biosynthesis of amino acids, aminoacyl-tRNA biosynthesis, cyanoamino acid metabolism, phenylpropanoid biosynthesis, and alanine, aspartate and glutamate metabolism. Notably, key genes involved in phenylpropanoid biosynthesis—<i>Rr4CLs</i>, <i>RrHCTs</i>, <i>RrBRT1</i> and <i>RrREF1</i>—were significantly upregulated during both the half-opening (BB) and full-opening (BQ) stages. Transcription factor analysis further indicated that MYBs (e.g., LOC133710114, LOC133721239, LOC133741925, etc.) play a critical role in regulating bioactive metabolite dynamics during floral development. Promoter analysis revealed the presence of MYB cis-acting elements in genes associated with phenylpropanoid biosynthesis, suggesting direct regulatory interactions. These findings provide a molecular foundation for further research on bioactive metabolites in <i>R. rugosa</i>.</p>

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Integrated Metabolome and Transcriptome Analyses Reveal the Regulatory Network of Bioactive Metabolite Dynamics During Floral Development in Rosa rugosa

  • 期刊论文

  • 发表日期：

    2026年7月21日

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  • Wentao Jiang,
  • Chao Ma,
  • Yaoyao Li,
  • Dedong Min,
  • Yunguo Liu

摘要

Rosa rugosa Thunb. (R. rugosa) possesses considerable medicinal value and has attracted global interest. Given that bioactive metabolites may vary during floral development, we employed transcriptomic and metabolomic approaches to elucidate their regulatory mechanisms. A total of 205 differentially accumulated metabolites and 6,303 differentially expressed genes were identified. Integrated transcriptome and metabolome analysis revealed significant enrichment in five key metabolic pathways: biosynthesis of amino acids, aminoacyl-tRNA biosynthesis, cyanoamino acid metabolism, phenylpropanoid biosynthesis, and alanine, aspartate and glutamate metabolism. Notably, key genes involved in phenylpropanoid biosynthesis—Rr4CLs, RrHCTs, RrBRT1 and RrREF1—were significantly upregulated during both the half-opening (BB) and full-opening (BQ) stages. Transcription factor analysis further indicated that MYBs (e.g., LOC133710114, LOC133721239, LOC133741925, etc.) play a critical role in regulating bioactive metabolite dynamics during floral development. Promoter analysis revealed the presence of MYB cis-acting elements in genes associated with phenylpropanoid biosynthesis, suggesting direct regulatory interactions. These findings provide a molecular foundation for further research on bioactive metabolites in R. rugosa.