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<p>bHLH transcription factors play vital roles in cold stress signaling pathways. In this study, a cabbage bHLH gene, designated <i>BobHLH15</i>, was identified and functionally characterized. Expression analysis showed that <i>BobHLH15</i> was induced by cold stress, particularly in leaf tissues, with transcript levels peaking at 24 h post-treatment. Subcellular localization assays confirmed that the BobHLH15 protein localized in the nucleus. Transgenic plants overexpressing <i>BobHLH15</i> showed elevated cold tolerance compared to wild-type plants. This improvement in stress resistance was associated with decreased accumulation of reactive oxygen species (ROS), increased antioxidant enzyme activities, elevated proline levels, and reduced malondialdehyde (MDA) content. Moreover, these transgenic lines demonstrated upregulated expression of cold-responsive genes, including C-repeat binding factors (<i>CBFs</i>) and cold-regulated (<i>COR</i>) genes. Collectively, these results suggest that <i>BobHLH15</i> contributes to cold tolerance, accompanied by the upregulation of defense-related genes and enhanced antioxidant system activity, thereby highlighting its theoretical potential for developing cold-tolerant varieties of <i>Brassica oleracea</i>, though direct functional validation in cabbage remains necessary.</p>

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Overexpression of Cabbage BobHLH15 Enhances Cold Tolerance in Arabidopsis Thaliana

  • 期刊论文

  • 发表日期：

    2026年7月29日

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  • Muhammad Adnan Raza,
  • Chen jing,
  • Hube Ali,
  • Song Jianghua

摘要

bHLH transcription factors play vital roles in cold stress signaling pathways. In this study, a cabbage bHLH gene, designated BobHLH15, was identified and functionally characterized. Expression analysis showed that BobHLH15 was induced by cold stress, particularly in leaf tissues, with transcript levels peaking at 24 h post-treatment. Subcellular localization assays confirmed that the BobHLH15 protein localized in the nucleus. Transgenic plants overexpressing BobHLH15 showed elevated cold tolerance compared to wild-type plants. This improvement in stress resistance was associated with decreased accumulation of reactive oxygen species (ROS), increased antioxidant enzyme activities, elevated proline levels, and reduced malondialdehyde (MDA) content. Moreover, these transgenic lines demonstrated upregulated expression of cold-responsive genes, including C-repeat binding factors (CBFs) and cold-regulated (COR) genes. Collectively, these results suggest that BobHLH15 contributes to cold tolerance, accompanied by the upregulation of defense-related genes and enhanced antioxidant system activity, thereby highlighting its theoretical potential for developing cold-tolerant varieties of Brassica oleracea, though direct functional validation in cabbage remains necessary.