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<p>Strigolactone (SL) has shown potential to enhance drought tolerance in various crops, yet its role in buckwheat remains unexplored. In this study, we investigated the impact of exogenous SL application on two contrasting genotypes, BWS25 (drought-tolerant) and BWS43 (drought-sensitive), under PEG-induced drought conditions, with emphasis on root system response. Our results showed that application of 6 µM GR24 (a Synthetic SL analog) improved growth parameters, relative water content, and stomatal and root morphology, especially in the drought-sensitive genotype. Furthermore, the SL application under drought stress (DT + SL) significantly increased shoot length by 29%, plant biomass by 183% and leaf area by 38% in BWS43 compared to drought-tolerant BWS25. The SL treatment mitigated drought-induced oxidative stress by improving antioxidant enzyme activities (superoxide dismutase, catalase, peroxidase, and glutathione reductase) and modulating osmolyte accumulation (phenolics, anthocyanins, and sugars). Furthermore, SL improved root parameters, including root length by 21%, surface area by 38%, root volume by 59%, root tips by 40%, perimeter, and number of roots in the sensitive genotype. Gene expression analysis of key root-associated and cell wall modification genes, such as <i>anthocyaninless 2</i> (<i>ANL2</i>) gene, <i>UDP-D-xylose 4-epimerase</i> (<i>MUR4</i>) gene, <i>microtubule stability-encoding alpha-tubulin 3</i> (<i>TUA3</i>), <i>trichome birefringence-like 33</i> (<i>TBL33</i>) gene, <i>ATPMEPCRB</i> gene, <i>glycine-rich protein</i> (<i>GRP</i>), showed altered expression, further emphasizing the role of SL in structural and functional integrity of the plant system under stress conditions. The present study highlights SL as a potential priming agent for developing drought resilience in buckwheat, a strategy for sustainable agricultural practices in water-deficient regions.</p>

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Strigolactone Induces Genotype-Specific Root Remodelling and Drought Stress Response in Buckwheat Under In Vitro Conditions

  • 期刊论文

  • 发表日期：

    2026年8月4日

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  • Showkat Ahmad Bhat,
  • Rayees Ahmad Rather,
  • Aijaz Ahmad Wani,
  • Aadil Yousuf Tantray,
  • Riffat John

摘要

Strigolactone (SL) has shown potential to enhance drought tolerance in various crops, yet its role in buckwheat remains unexplored. In this study, we investigated the impact of exogenous SL application on two contrasting genotypes, BWS25 (drought-tolerant) and BWS43 (drought-sensitive), under PEG-induced drought conditions, with emphasis on root system response. Our results showed that application of 6 µM GR24 (a Synthetic SL analog) improved growth parameters, relative water content, and stomatal and root morphology, especially in the drought-sensitive genotype. Furthermore, the SL application under drought stress (DT + SL) significantly increased shoot length by 29%, plant biomass by 183% and leaf area by 38% in BWS43 compared to drought-tolerant BWS25. The SL treatment mitigated drought-induced oxidative stress by improving antioxidant enzyme activities (superoxide dismutase, catalase, peroxidase, and glutathione reductase) and modulating osmolyte accumulation (phenolics, anthocyanins, and sugars). Furthermore, SL improved root parameters, including root length by 21%, surface area by 38%, root volume by 59%, root tips by 40%, perimeter, and number of roots in the sensitive genotype. Gene expression analysis of key root-associated and cell wall modification genes, such as anthocyaninless 2 (ANL2) gene, UDP-D-xylose 4-epimerase (MUR4) gene, microtubule stability-encoding alpha-tubulin 3 (TUA3), trichome birefringence-like 33 (TBL33) gene, ATPMEPCRB gene, glycine-rich protein (GRP), showed altered expression, further emphasizing the role of SL in structural and functional integrity of the plant system under stress conditions. The present study highlights SL as a potential priming agent for developing drought resilience in buckwheat, a strategy for sustainable agricultural practices in water-deficient regions.