Scholarlink

<p>Mechanistic insights into bioinoculant mediated improvement in the nutritional and organoleptic quality of cherry tomato fruits attributed to fruit microbiome modulation, necessitates investigation through high-throughput technologies. The inoculation of a plant growth promoting cyanobacterium <i>Anabaena laxa</i> RPAN8 through seed priming + soil drenching + foliar application significantly improved the fruit lycopene and phenolic content by 88.8 and 7.13% respectively. The inoculation of <i>Enterobacter cloacae</i> M2 (a native fruit mesocarpic bacterial isolate) as foliar application significantly enhanced the lycopene content by 57.7%, but reduced the ascorbic acid content by 42.8%. Shotgun metagenomics of red-ripe fruits revealed the enrichment of <i>Filobasidium floriforme</i>,<i> Synchytrium microbalum</i>, <i>Volvox carteri</i> and <i>Coccomyxa subellipsoidea</i> in the fruits of <i>A. laxa</i> treatment and predicted their functional associations with isoprenoid biosynthesis and phenylpropanoid precursor biosynthesis. Similarly, enriched members of <i>Brevundimonas</i> sp. and <i>Gluconacetobacter liquefaciens</i> in the fruits of <i>E. cloacae</i> treatment were functionally associated with aspartate/chorismate metabolism and pectin degradation respectively. <i>A. laxa</i> treatment was associated with the enrichment of microbial taxa functionally linked to the biosynthetic pathways of lycopene and phenolics, favouring higher accumulation. The reduction in ascorbic acid content under <i>E. cloacae</i> treatment may be linked to the enrichment of microbial functions associated with pectin degradation, while improved lycopene content was attributed to isoprenoid precursors, although this relationship requires further experimental validation. The interplay of microbial intervention (<i>Anabaena laxa</i> or <i>Enterobacter cloacae</i>) with tomato fruit microbiome triggered differential enrichment of native microbial species, suggesting possible functional associations with enhanced fruit quality traits.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Metagenomic Insights into the Fruit Microbiome and Quality Attributes of Cherry Tomato Mediated by Microbial Inoculation

  • 期刊论文

  • 发表日期：

    2026年7月21日

查看全文
  • Deepti Varsha,
  • Vishal S. Somvanshi,
  • Akanksha Bhardwaj,
  • Shalini Gaur Rudra,
  • Roli Budhwar,
  • Anita Tripathi,
  • Radha Prasanna

摘要

Mechanistic insights into bioinoculant mediated improvement in the nutritional and organoleptic quality of cherry tomato fruits attributed to fruit microbiome modulation, necessitates investigation through high-throughput technologies. The inoculation of a plant growth promoting cyanobacterium Anabaena laxa RPAN8 through seed priming + soil drenching + foliar application significantly improved the fruit lycopene and phenolic content by 88.8 and 7.13% respectively. The inoculation of Enterobacter cloacae M2 (a native fruit mesocarpic bacterial isolate) as foliar application significantly enhanced the lycopene content by 57.7%, but reduced the ascorbic acid content by 42.8%. Shotgun metagenomics of red-ripe fruits revealed the enrichment of Filobasidium floriforme, Synchytrium microbalum, Volvox carteri and Coccomyxa subellipsoidea in the fruits of A. laxa treatment and predicted their functional associations with isoprenoid biosynthesis and phenylpropanoid precursor biosynthesis. Similarly, enriched members of Brevundimonas sp. and Gluconacetobacter liquefaciens in the fruits of E. cloacae treatment were functionally associated with aspartate/chorismate metabolism and pectin degradation respectively. A. laxa treatment was associated with the enrichment of microbial taxa functionally linked to the biosynthetic pathways of lycopene and phenolics, favouring higher accumulation. The reduction in ascorbic acid content under E. cloacae treatment may be linked to the enrichment of microbial functions associated with pectin degradation, while improved lycopene content was attributed to isoprenoid precursors, although this relationship requires further experimental validation. The interplay of microbial intervention (Anabaena laxa or Enterobacter cloacae) with tomato fruit microbiome triggered differential enrichment of native microbial species, suggesting possible functional associations with enhanced fruit quality traits.