Scholarlink

<p>Phytic acid (PA), though limits nutrient bioavailability, plays a crucial role in seed development and stress responses. Although several low-phytate mutants with reduced PA have been developed, their effects on seed germination, vigour, and physio-biochemical properties remain largely unexplored. Here, we aimed to assess the impact of reduced PA on seed germination, vigour, and physio-biochemical properties. A set of 70 inbreds encompassing <i>lpa1-</i> and <i>lpa2</i>-based single (<i>lpa1</i> and <i>lpa2</i>), double (<i>lpa1lpa2</i>) mutant maize inbreds and their wild-type parents were evaluated in the field. Reduction in PA was prominent in single and double mutants, ranging from 28 to 41% (28%, 35% and 41% reduction in <i>lpa1</i>, <i>lpa2</i> and double mutant lines, respectively). Further, genotypes exhibited wide genetic variation for all traits related to germination, vigour, and physio-biochemical properties. <i>lpa1</i>-based inbred lines exhibited comparable germination, vigour index-I &amp; -II, dehydrogenase activity, and electric conductivity to wild-type inbreds. The <i>lpa2</i> and double mutant lines had minimally reduced germination (upto 6%), 17 to 27% higher esterase activity and 12 to 25% greater ion leachability than wild types. Among mutants, <i>lpa2</i> and double mutants showed comparable performance for most of the parameters. Significantly higher superoxide dismutase (14 to 23%) and peroxidase (12 to 25%) activity in single and double mutants than wild types highlighted superior reactive oxygen species (ROS) management. These findings demonstrated no major adverse effects of PA reduction on seed quality and vigour in <i>lpa1</i> and <i>lpa2</i>-based single and double mutant inbreds.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Minimal Adverse Effects of lpa1 and lpa2 Mutations on Seed Germination and Vigour in Low-Phytate Maize

  • 期刊论文

  • 发表日期：

    2026年5月27日

查看全文
  • Vinay Rojaria,
  • Ashvinkumar Katral,
  • Sudipta Basu,
  • Rajkumar Uttamrao Zunjare,
  • Govinda Rai Sarma,
  • Vinay Bhatt,
  • Atul Kumar,
  • Ashwani Kumar,
  • Gyanendra Singh,
  • Firoz Hossain,
  • Vignesh Muthusamy

摘要

Phytic acid (PA), though limits nutrient bioavailability, plays a crucial role in seed development and stress responses. Although several low-phytate mutants with reduced PA have been developed, their effects on seed germination, vigour, and physio-biochemical properties remain largely unexplored. Here, we aimed to assess the impact of reduced PA on seed germination, vigour, and physio-biochemical properties. A set of 70 inbreds encompassing lpa1- and lpa2-based single (lpa1 and lpa2), double (lpa1lpa2) mutant maize inbreds and their wild-type parents were evaluated in the field. Reduction in PA was prominent in single and double mutants, ranging from 28 to 41% (28%, 35% and 41% reduction in lpa1, lpa2 and double mutant lines, respectively). Further, genotypes exhibited wide genetic variation for all traits related to germination, vigour, and physio-biochemical properties. lpa1-based inbred lines exhibited comparable germination, vigour index-I & -II, dehydrogenase activity, and electric conductivity to wild-type inbreds. The lpa2 and double mutant lines had minimally reduced germination (upto 6%), 17 to 27% higher esterase activity and 12 to 25% greater ion leachability than wild types. Among mutants, lpa2 and double mutants showed comparable performance for most of the parameters. Significantly higher superoxide dismutase (14 to 23%) and peroxidase (12 to 25%) activity in single and double mutants than wild types highlighted superior reactive oxygen species (ROS) management. These findings demonstrated no major adverse effects of PA reduction on seed quality and vigour in lpa1 and lpa2-based single and double mutant inbreds.