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<p>The impact of waterlogging (WL) stress on crop growth and productivity is severe disrupting root development and inducing oxidative damage. The study is examining whether melatonin (MT) seed priming can be effective in promoting adventitious roots formation in tolerant genotype SSB-797 of Vigna unguiculata in response to WL conditions. The optimal time for MT priming was discovered to be 6&#xa0;h, then seeds were primed with water or 100 µmol MT. Seedlings were subjected to control (non-stressed), waterlogged (stressed), and PEG treatment (negative control) conditions. MT-primed seedlings showed increased expression of antioxidant genes (SOD, POD, and CAT) during PEG-induced drought stress, proving that MT can help reduce the stress. The phylogenetic analysis of 30 polyamine biosynthetic genes classified them into three groups, suggesting that structural differences may be involved in preventing different stresses. Conserved domain analysis revealed the presence of the “Spermine_synt_N” and “Adomet_Mtases” domains in SPDS and SPMS proteins, and the “Amino_oxidase” domain in all polyamine oxidase genes. RNA-seq transcriptional atlas analysis of <i>V. unguiculata</i> cv. IT97K-499-35 revealed the tissue-specific expression patterns. Transcript level assessment of candidate genes (<i>Vun/NR</i>,<i> Vun/NOS</i>,<i> Vun/SPDS-1</i>,<i> Vun/PO-3</i>, and <i>Vun/ADC</i>) indicated a significant response to stress too. According to the findings, MT was able to boost adventitious root development under WL by modifying polyamine (PA) biosynthesis and antioxidant defense pathways.</p>

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Melatonin Improves Waterlogging Tolerance in Cowpea Seedlings by Promoting Adventitious Roots, Regulating Polyamines and Nitric Oxide Encoding Genes

  • 期刊论文

  • 发表日期：

    2026年5月13日

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  • Srijan Yadav,
  • Dhirendra Kumar Singh,
  • Akanksha Maurya,
  • Priyanka Kaundal,
  • Divya Singh,
  • Mukul Kumar,
  • Chandra Mohan Singh

摘要

The impact of waterlogging (WL) stress on crop growth and productivity is severe disrupting root development and inducing oxidative damage. The study is examining whether melatonin (MT) seed priming can be effective in promoting adventitious roots formation in tolerant genotype SSB-797 of Vigna unguiculata in response to WL conditions. The optimal time for MT priming was discovered to be 6 h, then seeds were primed with water or 100 µmol MT. Seedlings were subjected to control (non-stressed), waterlogged (stressed), and PEG treatment (negative control) conditions. MT-primed seedlings showed increased expression of antioxidant genes (SOD, POD, and CAT) during PEG-induced drought stress, proving that MT can help reduce the stress. The phylogenetic analysis of 30 polyamine biosynthetic genes classified them into three groups, suggesting that structural differences may be involved in preventing different stresses. Conserved domain analysis revealed the presence of the “Spermine_synt_N” and “Adomet_Mtases” domains in SPDS and SPMS proteins, and the “Amino_oxidase” domain in all polyamine oxidase genes. RNA-seq transcriptional atlas analysis of V. unguiculata cv. IT97K-499-35 revealed the tissue-specific expression patterns. Transcript level assessment of candidate genes (Vun/NR, Vun/NOS, Vun/SPDS-1, Vun/PO-3, and Vun/ADC) indicated a significant response to stress too. According to the findings, MT was able to boost adventitious root development under WL by modifying polyamine (PA) biosynthesis and antioxidant defense pathways.