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<p>The CLE (CLAVATA3/Embryo surrounding region) family is one of the largest peptide families in plants, playing a crucial role in growth, development, and stress responses. Despite its significance, the biological functions of CLE peptides in cotton remain largely unexplored. In this study, we systematically analyzed the sequence characteristics, evolutionary relationships, and expression patterns of CLE family members in upland cotton, identifying 74 <i>CLE</i> genes and classifying them into five groups based on conserved motifs. Furthermore, we investigated the functional roles of 11 GhCLE (GhCLE6, GhCLE32, GhCLE39, GhCLE41, GhCLE42, GhCLE45, GhCLE47, GhCLE48, GhCLE49, GhCLE51, and GhCLE63) in shaping cotton root architecture. Our results demonstrate that, except for GhCLE51, exogenous application of the other 10 GhCLE peptides significantly inhibited primary root growth while promoting lateral root development in both cotton and <i>Arabidopsis</i>, with GhCLE47 exhibiting the most pronounced effect. Further studies suggest that GhCLEs modulate IAA signals in cotton root. Overall, this study highlights the essential role of GhCLEs in plant root morphogenesis and provides new insights into the regulatory mechanisms underlying cotton root growth and development.</p>

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Functional Characterization of CLE Peptides in Cotton Root Development Reveals GhCLE47 as a Key Regulator of Auxin-Mediated Root Architecture

  • 期刊论文

  • 发表日期：

    2026年4月13日

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  • Gai-Yuan Hu,
  • Peng Liao,
  • Xin-Yao Jiang,
  • Ying-Chao Tang,
  • Ting-Wan Li,
  • Yang Li,
  • Fu-Chun Xu,
  • Zhen-Nan Zhang

摘要

The CLE (CLAVATA3/Embryo surrounding region) family is one of the largest peptide families in plants, playing a crucial role in growth, development, and stress responses. Despite its significance, the biological functions of CLE peptides in cotton remain largely unexplored. In this study, we systematically analyzed the sequence characteristics, evolutionary relationships, and expression patterns of CLE family members in upland cotton, identifying 74 CLE genes and classifying them into five groups based on conserved motifs. Furthermore, we investigated the functional roles of 11 GhCLE (GhCLE6, GhCLE32, GhCLE39, GhCLE41, GhCLE42, GhCLE45, GhCLE47, GhCLE48, GhCLE49, GhCLE51, and GhCLE63) in shaping cotton root architecture. Our results demonstrate that, except for GhCLE51, exogenous application of the other 10 GhCLE peptides significantly inhibited primary root growth while promoting lateral root development in both cotton and Arabidopsis, with GhCLE47 exhibiting the most pronounced effect. Further studies suggest that GhCLEs modulate IAA signals in cotton root. Overall, this study highlights the essential role of GhCLEs in plant root morphogenesis and provides new insights into the regulatory mechanisms underlying cotton root growth and development.