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<p>In this study, we identified 87 members of the <i>SAUR</i> gene family within the <i>Populus simonii</i> genome, a notably extensive family with a central role in auxin responses. Phylogenetic analysis classified these genes into five distinct subfamilies. Chromosomal mapping revealed an uneven distribution of <i>PsSAUR</i> genes across 19 chromosomes, with notable clustering on chromosomes 4 and 9. Structural analysis indicated that members of the same subfamily share highly conserved protein domain architectures and motif organizations. Promoter analysis identified various cis-acting elements associated with light, phytohormones, abiotic stress, and general growth and development. Transcriptome profiling revealed tissue-specific and stress-responsive expression patterns, underscoring the transcriptional plasticity of <i>PsSAUR</i> genes in developmental regulation and environmental adaptation. These findings were further validated through RT-qPCR analysis, confirming the differential expression of selected <i>PsSAURs</i>. Notably, <i>PsSAUR66</i> was identified as a key gene in the interaction network. Functional validation using transient Pro<i>PsSAUR66</i>:GUS assays in tobacco demonstrated that its promoter activity is strongly induced by heat, cold, and salt stresses. These findings provide novel insights into the regulatory roles of <i>PsSAUR</i> genes and lay the groundwork for further functional characterization in woody plants.</p>

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Small Auxin-up RNA (SAUR) Gene Family in Populus simonii: A Comprehensive Study on Gene Identification and Functional Analysis

  • 期刊论文

  • 发表日期：

    2026年2月16日

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  • Xiaoyu Xie,
  • Xinru Bi,
  • Wanying Chen,
  • Lu Han,
  • Junbo Chen,
  • Yishuang Ren,
  • Guowei Yao,
  • Jinwang Zhang,
  • Hongtao Yuan,
  • Yi Hao,
  • Sitong Du,
  • Yunchang Zhang,
  • Kewei Cai,
  • Xiyang Zhao

摘要

In this study, we identified 87 members of the SAUR gene family within the Populus simonii genome, a notably extensive family with a central role in auxin responses. Phylogenetic analysis classified these genes into five distinct subfamilies. Chromosomal mapping revealed an uneven distribution of PsSAUR genes across 19 chromosomes, with notable clustering on chromosomes 4 and 9. Structural analysis indicated that members of the same subfamily share highly conserved protein domain architectures and motif organizations. Promoter analysis identified various cis-acting elements associated with light, phytohormones, abiotic stress, and general growth and development. Transcriptome profiling revealed tissue-specific and stress-responsive expression patterns, underscoring the transcriptional plasticity of PsSAUR genes in developmental regulation and environmental adaptation. These findings were further validated through RT-qPCR analysis, confirming the differential expression of selected PsSAURs. Notably, PsSAUR66 was identified as a key gene in the interaction network. Functional validation using transient ProPsSAUR66:GUS assays in tobacco demonstrated that its promoter activity is strongly induced by heat, cold, and salt stresses. These findings provide novel insights into the regulatory roles of PsSAUR genes and lay the groundwork for further functional characterization in woody plants.