Scholarlink

<p>Drought stress is a significant global environmental challenge that threatens food security, affecting ~ 66% of arable land and resulting in economic losses estimated at USD 37&#xa0;billion, with further increases anticipated due to climate change. <i>Cajanus cajan</i> (pigeonpea), a widely cultivated grain legume in the semi-arid tropics, often experiences 40–50% yield reduction under drought. Understanding the genetic basis of drought tolerance is critical for selecting diverse parental lines and designing effective crop improvement programs. In this study, 47 pigeonpea genotypes were evaluated under well-watered (WW; 60–70% field capacity) and drought-stressed (DS; 18–20% field capacity) conditions, with drought imposed by withholding irrigation from 80 to 120 days after sowing, corresponding to the flowering to pod-setting stage, in a controlled greenhouse. Drought stress significantly reduced most morpho-physiological traits (2.8–68.8%), while vapour pressure deficit and proline accumulation increased by 1–1.5-fold. High-throughput phenomic traits, including digital area (projected shoot area), convex hull area (CHA), calliper length (CL), and near-infrared reflectance (NIR), effectively distinguished drought-tolerant from sensitive genotypes. Nine genotypes, <i>viz.</i>, ICPX140196-B-1, ICPX140203-B-1, ICPX140213-B-3, ICPX140203-B-1-5, ICPX140203-B-2, GRG-152, ICPX140205-B-4, ICPX140217-B-1, and ICPX140188-B-3, were identified as drought-tolerant, exhibiting superior photosynthetic efficiency (Net CO₂ assimilation rate), favourable tissue water status, and minimal grain yield reduction (&lt; 5%). Notably, leaf temperature was negatively associated with grain yield. Moreover, photosynthetic parameters showed significant associations with grain yield under stress and exhibited high genetic variability (10–46%) with moderate heritability, highlighting their utility as selection indices. These findings demonstrate the potential of integrating high-throughput phenomics with conventional morpho-physiological traits for the rapid and precise identification of drought-tolerant pigeonpea genotypes, providing insights for introgression breeding and genetic enhancement programs aimed at improving drought tolerance.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Leveraging High-Throughput Phenomics and Morpho-Physiological Traits for Selecting Drought-Tolerant Pigeonpea [Cajanus cajan (L.) Millspaugh] Genotypes

  • 期刊论文

  • 发表日期：

    2026年1月19日

查看全文
  • Prasad,
  • S. Muniswamy,
  • P. S. Basavaraj,
  • Anuja Gangurde,
  • Rohit Babar,
  • Shweta Shinde,
  • K. M. Boraiah,
  • C. B. Harisha,
  • H. M. Halli,
  • Dasari Sreekanth,
  • Praveen Kumar,
  • C. Laxuman,
  • T. C. Suma,
  • Prakash Kuchanur,
  • Prakash Gangashetty,
  • K. Sammi Reddy

摘要

Drought stress is a significant global environmental challenge that threatens food security, affecting ~ 66% of arable land and resulting in economic losses estimated at USD 37 billion, with further increases anticipated due to climate change. Cajanus cajan (pigeonpea), a widely cultivated grain legume in the semi-arid tropics, often experiences 40–50% yield reduction under drought. Understanding the genetic basis of drought tolerance is critical for selecting diverse parental lines and designing effective crop improvement programs. In this study, 47 pigeonpea genotypes were evaluated under well-watered (WW; 60–70% field capacity) and drought-stressed (DS; 18–20% field capacity) conditions, with drought imposed by withholding irrigation from 80 to 120 days after sowing, corresponding to the flowering to pod-setting stage, in a controlled greenhouse. Drought stress significantly reduced most morpho-physiological traits (2.8–68.8%), while vapour pressure deficit and proline accumulation increased by 1–1.5-fold. High-throughput phenomic traits, including digital area (projected shoot area), convex hull area (CHA), calliper length (CL), and near-infrared reflectance (NIR), effectively distinguished drought-tolerant from sensitive genotypes. Nine genotypes, viz., ICPX140196-B-1, ICPX140203-B-1, ICPX140213-B-3, ICPX140203-B-1-5, ICPX140203-B-2, GRG-152, ICPX140205-B-4, ICPX140217-B-1, and ICPX140188-B-3, were identified as drought-tolerant, exhibiting superior photosynthetic efficiency (Net CO₂ assimilation rate), favourable tissue water status, and minimal grain yield reduction (< 5%). Notably, leaf temperature was negatively associated with grain yield. Moreover, photosynthetic parameters showed significant associations with grain yield under stress and exhibited high genetic variability (10–46%) with moderate heritability, highlighting their utility as selection indices. These findings demonstrate the potential of integrating high-throughput phenomics with conventional morpho-physiological traits for the rapid and precise identification of drought-tolerant pigeonpea genotypes, providing insights for introgression breeding and genetic enhancement programs aimed at improving drought tolerance.

Graphical Abstract