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<p>Blue honeysuckle (<i>Lonicera caerulea</i> L.) fruits are rich in anthocyanins, vitamins, and other bioactive compounds, offering high nutritional and health value. However, the fruit is prone to premature abscission during ripening, which severely affects production yield and economic benefits, thereby limiting the development of the industry. This study investigates the effect of the plant growth regulator gibberellin (GA₃) on fruit abscission in blue honeysuckle and explores the underlying regulatory mechanisms. Using the abscission-prone cultivar ‘HSY’ as the experimental material, we analyzed the anatomical structure of the fruit abscission zone (FAZ), cell wall-degrading enzyme activity, and gene expression following GA₃ application. Exogenous GA₃ treatment significantly reduced the fruit drop rate in ‘HSY’, accompanied by decreased activities of key cell wall-degrading enzymes including cellulase (Cx), β-glucosidase (β-glu), polygalacturonase (PG), and pectin methylesterase (PME), indicating the crucial role of these enzymes in regulating abscission. Transcriptomic analysis revealed that the differentially expressed genes (DEGs) involved in abscission are mainly associated with plant hormone signal transduction, pentose and glucuronate interconversion, and phenylpropanoid biosynthesis pathways. The regulatory mechanisms by which exogenous GA₃ mitigates fruit abscission can therefore be summarized into two major aspects: cell wall metabolism in the abscission zone and plant hormone synthesis and signaling. A total of 76 key DEGs were identified, and the expression patterns of 12 candidate genes were further validated via quantitative real-time PCR (qRT-PCR), confirming the reliability of the transcriptome data and revealing their expression profiles in response to hormone treatment. This study provides theoretical insights into the mechanisms of fruit abscission in blue honeysuckle, and offers a basis for strategies aimed at extending the fruit’s on-tree storage life.</p>

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Transcriptomic and Physiological Insights into GA₃-Mediated Regulation of Fruit Abscission in Lonicera Caerulea L

  • 期刊论文

  • 发表日期：

    2026年1月9日

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  • Kailin Shao,
  • Jing Chen,
  • Bingbing Ren,
  • Dong Qin,
  • Xueting Wang,
  • Chunlin Fu,
  • Ran Gu,
  • Jiacheng Li,
  • Zhonglin Jia,
  • Zhenyu Li,
  • Junwei Huo,
  • Huixin Gang

摘要

Blue honeysuckle (Lonicera caerulea L.) fruits are rich in anthocyanins, vitamins, and other bioactive compounds, offering high nutritional and health value. However, the fruit is prone to premature abscission during ripening, which severely affects production yield and economic benefits, thereby limiting the development of the industry. This study investigates the effect of the plant growth regulator gibberellin (GA₃) on fruit abscission in blue honeysuckle and explores the underlying regulatory mechanisms. Using the abscission-prone cultivar ‘HSY’ as the experimental material, we analyzed the anatomical structure of the fruit abscission zone (FAZ), cell wall-degrading enzyme activity, and gene expression following GA₃ application. Exogenous GA₃ treatment significantly reduced the fruit drop rate in ‘HSY’, accompanied by decreased activities of key cell wall-degrading enzymes including cellulase (Cx), β-glucosidase (β-glu), polygalacturonase (PG), and pectin methylesterase (PME), indicating the crucial role of these enzymes in regulating abscission. Transcriptomic analysis revealed that the differentially expressed genes (DEGs) involved in abscission are mainly associated with plant hormone signal transduction, pentose and glucuronate interconversion, and phenylpropanoid biosynthesis pathways. The regulatory mechanisms by which exogenous GA₃ mitigates fruit abscission can therefore be summarized into two major aspects: cell wall metabolism in the abscission zone and plant hormone synthesis and signaling. A total of 76 key DEGs were identified, and the expression patterns of 12 candidate genes were further validated via quantitative real-time PCR (qRT-PCR), confirming the reliability of the transcriptome data and revealing their expression profiles in response to hormone treatment. This study provides theoretical insights into the mechanisms of fruit abscission in blue honeysuckle, and offers a basis for strategies aimed at extending the fruit’s on-tree storage life.