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<p>Cotton (<i>Gossypium</i> spp.) fiber is one of the most important natural fiber sources. However, its yield and quality are severely limited by drought stress. Our previous study identified <i>GsWRKY70</i> as a hub gene regulating the drought response in the wild cotton <i>G. stocksii</i>, but its functional mechanisms remained unclear. This study revealed that heterologous overexpression of <i>GsWRKY70</i> in <i>Arabidopsis thaliana</i> significantly suppressed early seed germination and reduced root growth by 25–39% under mannitol mimicked drought conditions. Furthermore, transgenic <i>Arabidopsis</i> plants exhibited a significantly lower survival rate (37–47%) compared to the wild type (77%) under drought conditions. Subsequent analyses revealed that <i>GsWRKY70</i> overexpression resulted in increased stomatal density, reduced stomatal size, and larger stomatal apertures under drought stress. Stressed transgenic plants also accumulated higher levels of reactive oxygen species and showed significantly reduced activities of predominant antioxidant enzymes. These findings suggest that GsWRKY70 acts as a negative regulator of drought tolerance, which was possibly mediated by regulating stomatal closure and modulating antioxidant enzyme activity. Our results also revealed a dual role of GsWRKY70 in regulating abscisic acid (ABA) sensitivity. In contrast to suppressing ABA sensitivity under drought stress, it possibly regulates seed germination and root growth by enhancing ABA sensitivity. This study enhanced our understanding of the drought tolerance mechanisms in cotton species and will promote the future utilization of wild cotton genetic resources in improving the drought tolerance of cultivated varieties.</p>

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Identification of WRKY70 as a Negative Regulator of Drought Tolerance in Wild Cotton Gossypium Stocksii

  • 期刊论文

  • 发表日期：

    2025年12月5日

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  • Haotian Zhou,
  • Shengbo Wang,
  • Jialin Chen,
  • Dongliang Yu

摘要

Cotton (Gossypium spp.) fiber is one of the most important natural fiber sources. However, its yield and quality are severely limited by drought stress. Our previous study identified GsWRKY70 as a hub gene regulating the drought response in the wild cotton G. stocksii, but its functional mechanisms remained unclear. This study revealed that heterologous overexpression of GsWRKY70 in Arabidopsis thaliana significantly suppressed early seed germination and reduced root growth by 25–39% under mannitol mimicked drought conditions. Furthermore, transgenic Arabidopsis plants exhibited a significantly lower survival rate (37–47%) compared to the wild type (77%) under drought conditions. Subsequent analyses revealed that GsWRKY70 overexpression resulted in increased stomatal density, reduced stomatal size, and larger stomatal apertures under drought stress. Stressed transgenic plants also accumulated higher levels of reactive oxygen species and showed significantly reduced activities of predominant antioxidant enzymes. These findings suggest that GsWRKY70 acts as a negative regulator of drought tolerance, which was possibly mediated by regulating stomatal closure and modulating antioxidant enzyme activity. Our results also revealed a dual role of GsWRKY70 in regulating abscisic acid (ABA) sensitivity. In contrast to suppressing ABA sensitivity under drought stress, it possibly regulates seed germination and root growth by enhancing ABA sensitivity. This study enhanced our understanding of the drought tolerance mechanisms in cotton species and will promote the future utilization of wild cotton genetic resources in improving the drought tolerance of cultivated varieties.