Scholarlink

<p>High temperature is a major abiotic factor that limits rice growth, physiological processes, and crop productivity. Thus, understanding the genetic mechanism and identifying novel genes implicated in high temperature tolerance is critical for developing climate-resilient rice cultivars. In this investigation, two rice genotypes, NERICA-L-44 (NL-44) and Uma, with varied tolerance to high temperature stress, were used to evaluate the physiological response and differentially expressed genes for high temperature adaptation (38–40&#xa0;°C). The high temperature tolerant, NL-44 showed lowest percentage reduction in stomatal conductance, photosynthetic rate, electron transport rate, Fv/Fm (photochemical efficiency), cell membrane stability and pollen viability under stress compared to high temperature sensitive variety, Uma. Comparative RNA sequencing at 30&#xa0;days of seedling stage found that NL-44 had 6034 differentially expressed genes while Uma had 4831 DEGs. Whereas, 6733 DEGs were shared between both NL-44 and Uma. In NL-44, 1939 genes were upregulated and 4794 genes were downregulated and Uma contributed 1899 upregulated and 4834 down regulated genes, among the whole set of shared DEGs. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and pathway analysis revealed that the DEGs of the high temperature-tolerant genotype were preferably enriched in the pyruvate, propanoate, glyoxylate and dicarboxylate metabolism. These pathways have inter links at gluconeogenesis, process involved in glucose synthesis during abiotic stress conditions. The expression studies of sugar signaling genes at grain filling stage revealed that, the increased expression of <i>OsTOR</i> and <i>OsHXK2</i> were observed in tolerant variety NL-44. These findings lay the groundwork for future research into the regulatory mechanisms of rice adaptation to high temperatures.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Comparative Transcriptome Analysis Uncovers Metabolic Pathways and Sugar Signalling Mechanisms Underpinning Heat Stress Tolerance in Rice Genotypes

  • 期刊论文

  • 发表日期：

    2025年12月10日

查看全文
  • Radha Beena,
  • Raghu Nalishma,
  • Rameswar Prasad Sah,
  • Sabarinathan Selvaraj,
  • A. G. Kiran,
  • Kunhikrishnan Hemalatha Dhanyalakshmi

摘要

High temperature is a major abiotic factor that limits rice growth, physiological processes, and crop productivity. Thus, understanding the genetic mechanism and identifying novel genes implicated in high temperature tolerance is critical for developing climate-resilient rice cultivars. In this investigation, two rice genotypes, NERICA-L-44 (NL-44) and Uma, with varied tolerance to high temperature stress, were used to evaluate the physiological response and differentially expressed genes for high temperature adaptation (38–40 °C). The high temperature tolerant, NL-44 showed lowest percentage reduction in stomatal conductance, photosynthetic rate, electron transport rate, Fv/Fm (photochemical efficiency), cell membrane stability and pollen viability under stress compared to high temperature sensitive variety, Uma. Comparative RNA sequencing at 30 days of seedling stage found that NL-44 had 6034 differentially expressed genes while Uma had 4831 DEGs. Whereas, 6733 DEGs were shared between both NL-44 and Uma. In NL-44, 1939 genes were upregulated and 4794 genes were downregulated and Uma contributed 1899 upregulated and 4834 down regulated genes, among the whole set of shared DEGs. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and pathway analysis revealed that the DEGs of the high temperature-tolerant genotype were preferably enriched in the pyruvate, propanoate, glyoxylate and dicarboxylate metabolism. These pathways have inter links at gluconeogenesis, process involved in glucose synthesis during abiotic stress conditions. The expression studies of sugar signaling genes at grain filling stage revealed that, the increased expression of OsTOR and OsHXK2 were observed in tolerant variety NL-44. These findings lay the groundwork for future research into the regulatory mechanisms of rice adaptation to high temperatures.