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<p>L-<i>M</i>yo-inositol-1-phosphate synthase (MIPS) catalyzes the rate-limiting step of <i>myo</i>-inositol (MI), a critical molecule involved in plant growth, development, and stress responses. However, the effects of MIPS on kiwifruit remain unclear. In this study, transgenic kiwifruit (<i>Actinidia chinensis</i> var. <i>deliciosa</i>) seedlings were generated via RNA interference (RNAi) of <i>AdMIPS1</i>. Relative to those of the wild type (WT), the expression level of <i>AdMIPS1</i> in the RNAi lines was decreased by 42–83%, and the MI content was concomitantly reduced by 89–97%. The RNAi lines showed an evident dwarf morphology, whereas their tolerance to drought or salt stress was relatively unchanged based on measurements of membrane leakage and antioxidative enzyme activities. Pathway enrichment analysis of metabolomics data from 90-day-acclimatized plants indicated significant changes in various metabolites, including flavonoids and ascorbic acid (AsA), in RNAi line 15 (RL15). The sucrose and raffinose contents of RL15 decreased to 78% and 9.9% of those in WT, respectively, while the glucose content increased by 1.2-fold after RNAi. The indole-3-acetic acid, gibberellin, and salicylic acid levels in RL15 decreased to 55%, 20%, and 40% of those in WT, respectively, while the jasmonic acid and abscisic acid levels were significantly increased in RL15. Analysis of the relative expression of genes involved in the biosynthesis of the metabolites mentioned above confirmed these effects at the transcription level. Overall, this study indicates that <i>AdMIPS1</i> RNAi-induced downregulation of MI in kiwifruit results in a complex modulation of sugars, growth-related and stress-related phytohormones, and antioxidants such as flavonoids and AsA.</p>

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Downregulation of the Myo-inositol-1-phosphate synthase Gene Represses the Growth and Alters the Metabolome of Kiwifruit Seedlings

  • 期刊论文

  • 发表日期：

    2025年12月2日

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  • Meng Cui,
  • Qifang Sun,
  • Dong Wu,
  • Yingbin Hao

摘要

L-Myo-inositol-1-phosphate synthase (MIPS) catalyzes the rate-limiting step of myo-inositol (MI), a critical molecule involved in plant growth, development, and stress responses. However, the effects of MIPS on kiwifruit remain unclear. In this study, transgenic kiwifruit (Actinidia chinensis var. deliciosa) seedlings were generated via RNA interference (RNAi) of AdMIPS1. Relative to those of the wild type (WT), the expression level of AdMIPS1 in the RNAi lines was decreased by 42–83%, and the MI content was concomitantly reduced by 89–97%. The RNAi lines showed an evident dwarf morphology, whereas their tolerance to drought or salt stress was relatively unchanged based on measurements of membrane leakage and antioxidative enzyme activities. Pathway enrichment analysis of metabolomics data from 90-day-acclimatized plants indicated significant changes in various metabolites, including flavonoids and ascorbic acid (AsA), in RNAi line 15 (RL15). The sucrose and raffinose contents of RL15 decreased to 78% and 9.9% of those in WT, respectively, while the glucose content increased by 1.2-fold after RNAi. The indole-3-acetic acid, gibberellin, and salicylic acid levels in RL15 decreased to 55%, 20%, and 40% of those in WT, respectively, while the jasmonic acid and abscisic acid levels were significantly increased in RL15. Analysis of the relative expression of genes involved in the biosynthesis of the metabolites mentioned above confirmed these effects at the transcription level. Overall, this study indicates that AdMIPS1 RNAi-induced downregulation of MI in kiwifruit results in a complex modulation of sugars, growth-related and stress-related phytohormones, and antioxidants such as flavonoids and AsA.