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<p><i>Brassica juncea</i> is an important oilseed crop that contributes to the agricultural economy worldwide. The huge yield loss caused by <i>Lipaphis erysimi</i> drew attention to mine its vital gene and develop aphid-resistant <i>Brassica</i>. This is the first report of the <i>L. erysimi</i> draft genome sequence and its functional annotation. A 203 vital target genes of <i>L. erysimi</i> were identified by subtractive genomics approach and were screened for the conserved domains to design the knockdown construct. The gene ontology (GO) analysis and key targets identified in <i>L. erysimi</i> revealed a high abundance of sequences associated with macromolecule metabolic processes (MMP). The plant-based insecticidal genes <i>chickpea lectin</i> (Accession: HM235917.1) and <i>Urdbean protease inhibitor</i> (Accession: KU382735.1) target the MMP of <i>L. erysimi</i>. Further, the <i>Brassica rapa</i>, <i>B. nigra</i> and <i>B. juncea</i> genomes were mined for candidate genes containing <i>CHPL</i> and <i>UPI</i> genes. Only one <i>CHPL</i> ortho-group was present in <i>Brassica</i> species but in a different clade whereas <i>UPI</i> orthologs were absent in <i>Brassica</i> sp. With this confirmation, two transgenic parental lines (T3) were crossed and advanced to the F3 population by progeny-to-row testing to get homozygous lines. The information derived from identified vital genes of aphids could be used to develop aphid-resistant crops.</p>

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Whole Genome Sequencing and Subtractive Genomics of Lipaphis erysimi: A Vital Pest of Brassica juncea

  • 期刊论文

  • 发表日期：

    2025年12月8日

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  • Deepanshu Jayaswal,
  • Ratna Prabha,
  • Kuldeep Kumar,
  • Deepesh Kumar,
  • Kuldip Jayaswall,
  • Archana Anokhe,
  • Arvind Nath Singh,
  • Sanjay Kumar,
  • Rekha Kansal

摘要

Brassica juncea is an important oilseed crop that contributes to the agricultural economy worldwide. The huge yield loss caused by Lipaphis erysimi drew attention to mine its vital gene and develop aphid-resistant Brassica. This is the first report of the L. erysimi draft genome sequence and its functional annotation. A 203 vital target genes of L. erysimi were identified by subtractive genomics approach and were screened for the conserved domains to design the knockdown construct. The gene ontology (GO) analysis and key targets identified in L. erysimi revealed a high abundance of sequences associated with macromolecule metabolic processes (MMP). The plant-based insecticidal genes chickpea lectin (Accession: HM235917.1) and Urdbean protease inhibitor (Accession: KU382735.1) target the MMP of L. erysimi. Further, the Brassica rapa, B. nigra and B. juncea genomes were mined for candidate genes containing CHPL and UPI genes. Only one CHPL ortho-group was present in Brassica species but in a different clade whereas UPI orthologs were absent in Brassica sp. With this confirmation, two transgenic parental lines (T3) were crossed and advanced to the F3 population by progeny-to-row testing to get homozygous lines. The information derived from identified vital genes of aphids could be used to develop aphid-resistant crops.