<p><i>Cymbidium ensifolium</i> is recognized as one of the most significant and widely cultivated ornamental plants within the Orchidaceae family. Its flowering period extends from June to September, out of alignment with China’s National Day, New Year’s Day, the Spring Festival, and other significant holidays, which poses challenges for the industry’s development. This study examined the growth rhythm of three-year-old <i>C. ensifolium</i> ‘Dongfang Honghe’ and determined that, without intervention, flower bud differentiation typically occurs from late April to early June, flower development from late May to mid-July, reaching peak flowering in mid-July and concluding by late August. The research further investigated the regulation of the flowering period of ‘Dongfang Honghe’, through temperature manipulation and the application of plant growth regulators. It was observed that decreasing treatment temperature resulted in a continuous delay of flowering phase, and varying concentrations of 6-Benzylaminopurine (6-BA) produced significant differences in both flowering time and flower quality. Additionally, through the application of temperature treatments and 6-BA, is feasible to achieve flowering on Labor Day, National Day, and New Year’s Day. Finally, utilizing transcriptome data from various stages of flower buds development in ‘Dongfang Honghe’, an identification and expression analysis of flowering-related genes was conducted. Preliminary findings suggest the involvement of three distinct pathways in the induction of flowering, facilitating the transition from vegetative to reproductive growth: the environmental temperature pathway, the heat stress pathway, and the gibberellin pathway. This study systematically explores flowering period control techniques based on the growth rhythm of ‘Dongfang Honghe’ and analyzes the regulatory pathways of flower induction, thereby establishing a foundation for the development of a flowering regulation technique system for <i>C. ensifolium</i>.</p>