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<p>Plants survive under the influence of various environmental factors. Drought is an abiotic stress factor that influences the growth and development of plants. Strigolactones (SL) are a new class of carotenoid-derived plant hormones that have fundamental effects on adaptation to various abiotic stresses. In addition, under drought stress, the mechanism of action of different SL concentrations in <i>Medicago truncatula</i>, the primary model plant for legume genomics research, remains unclear. In this study, foliar application of 1&#xa0;µM and 5&#xa0;µM SL analog GR24 was applied to drought-induced <i>Medicago truncatula,</i> and physiological analyses were performed at 24th and 48th hours following the application. When analyzing physiological outcomes, elevated levels of strigolactones (SLs) may mitigate stress-induced damage by enhancing osmotic adjustment and modulating the activities of antioxidant enzymes. Notably, in our findings, the application of 5&#xa0;µM SL demonstrated values closely aligned with those of the regular irrigation group in terms of both physiological parameters and photosynthetic capacity. RNA sequencing was conducted to analyze the gene-level responses to SL application at both concentrations and time points.. The results showed that 5&#xa0;µM treatment at 48&#xa0;h upregulated 2461 genes; involved in pathways such as "Biosynthesis of amino acids," "Oxidative phosphorylation," and the "Citrate cycle (TCA cycle)," in comparison to the 1&#xa0;µM SL treatment. This suggests that the ameliorative effects of SL administration are mainly related to the maintenance of energy metabolism and the synthesis of primary metabolites. Furthermore, SL administration may activate the amino acid biosynthesis pathway and preserve the building blocks required for protein synthesis, thereby contributing to the maintenance of osmotic balance and cellular homeostasis. The increased amount of SL in our study suggests that it may act as a positive regulator of abiotic stress in legume plants.</p>

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Transcriptional Effects of Foliar Spraying of Increasing Strigolactone on the Legume Model Medicago truncatula

  • 期刊论文

  • 发表日期：

    2025年11月18日

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  • Hande Morgil

摘要

Plants survive under the influence of various environmental factors. Drought is an abiotic stress factor that influences the growth and development of plants. Strigolactones (SL) are a new class of carotenoid-derived plant hormones that have fundamental effects on adaptation to various abiotic stresses. In addition, under drought stress, the mechanism of action of different SL concentrations in Medicago truncatula, the primary model plant for legume genomics research, remains unclear. In this study, foliar application of 1 µM and 5 µM SL analog GR24 was applied to drought-induced Medicago truncatula, and physiological analyses were performed at 24th and 48th hours following the application. When analyzing physiological outcomes, elevated levels of strigolactones (SLs) may mitigate stress-induced damage by enhancing osmotic adjustment and modulating the activities of antioxidant enzymes. Notably, in our findings, the application of 5 µM SL demonstrated values closely aligned with those of the regular irrigation group in terms of both physiological parameters and photosynthetic capacity. RNA sequencing was conducted to analyze the gene-level responses to SL application at both concentrations and time points.. The results showed that 5 µM treatment at 48 h upregulated 2461 genes; involved in pathways such as "Biosynthesis of amino acids," "Oxidative phosphorylation," and the "Citrate cycle (TCA cycle)," in comparison to the 1 µM SL treatment. This suggests that the ameliorative effects of SL administration are mainly related to the maintenance of energy metabolism and the synthesis of primary metabolites. Furthermore, SL administration may activate the amino acid biosynthesis pathway and preserve the building blocks required for protein synthesis, thereby contributing to the maintenance of osmotic balance and cellular homeostasis. The increased amount of SL in our study suggests that it may act as a positive regulator of abiotic stress in legume plants.