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<p>Phosphorus (P) deficiency adversely affects the photosynthetic efficiency, biomass accumulation, and yielding ability of wheat. In wheat, the knowledge on the genetics of phosphorous use efficiency (PUE) is limited, especially for photosynthesis related traits under low-P stress. Here, we phenotyped 124 bread wheat accessions for photosynthesis traits, biomass, yield, and index traits in pots with low and optimum P soil for two growing seasons. Under low-P stress, transpiration rate significantly increased (23.23% in Y1 and 25.42% in Y2) while net-assimilation rate (A), stomatal conductance (g<sub>s</sub>), biomass, instantaneous-water use efficiency (IWUE), yield, and harvest index (HI) significantly decreased. Trait correlations were generally stronger under optimum P than under low P. Frequency distribution and ANOVA revealed significant variation in all traits. Multi-locus genome-wide association study using 13,026 high-quality SNPs identified 13 major MTAs (phenotypic variation explained &gt; 10%); one MTA (AX-944540053) showing homology to <i>PSTOL1</i> locus in kasalath (traditional <i>aus</i>-type rice variety). Candidate gene (CG) and their promoter and expression analysis identified 12 putative-CGs which mainly encodes following protein/products- protein kinases, serine threonine protein kinases, glutathione, psbQ-like superfamily, and ferroportin-1. The identified cis-regulatory elements for the 12 potential CGs are associated with phytohormone synthesis, photosynthesis, abiotic stress responses, and PUE. The major MTAs identified can be converted into breeder friendly DNA markers for use in breeding wheat cultivars for improved photosynthesis and PUE after further validation using segregating biparental-mapping population.</p>

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Genetic Architecture of Photosynthesis and Yield Related Traits in Response To Low Phosphorus Stress in Wheat Using GWAS

  • 期刊论文

  • 发表日期：

    2025年11月3日

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  • Vijay Rajamanickam,
  • Gautam Saripalli,
  • Amitha Mithra Sevanthi,
  • Nisha Singh,
  • Vikas Kumar Singh,
  • Renu Pandey

摘要

Phosphorus (P) deficiency adversely affects the photosynthetic efficiency, biomass accumulation, and yielding ability of wheat. In wheat, the knowledge on the genetics of phosphorous use efficiency (PUE) is limited, especially for photosynthesis related traits under low-P stress. Here, we phenotyped 124 bread wheat accessions for photosynthesis traits, biomass, yield, and index traits in pots with low and optimum P soil for two growing seasons. Under low-P stress, transpiration rate significantly increased (23.23% in Y1 and 25.42% in Y2) while net-assimilation rate (A), stomatal conductance (gs), biomass, instantaneous-water use efficiency (IWUE), yield, and harvest index (HI) significantly decreased. Trait correlations were generally stronger under optimum P than under low P. Frequency distribution and ANOVA revealed significant variation in all traits. Multi-locus genome-wide association study using 13,026 high-quality SNPs identified 13 major MTAs (phenotypic variation explained > 10%); one MTA (AX-944540053) showing homology to PSTOL1 locus in kasalath (traditional aus-type rice variety). Candidate gene (CG) and their promoter and expression analysis identified 12 putative-CGs which mainly encodes following protein/products- protein kinases, serine threonine protein kinases, glutathione, psbQ-like superfamily, and ferroportin-1. The identified cis-regulatory elements for the 12 potential CGs are associated with phytohormone synthesis, photosynthesis, abiotic stress responses, and PUE. The major MTAs identified can be converted into breeder friendly DNA markers for use in breeding wheat cultivars for improved photosynthesis and PUE after further validation using segregating biparental-mapping population.