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<p>The magnesium transporter (MGT) gene family is known for transport activities and regulating responses to environmental stresses. Magnesium (Mg) is an essential nutrient required for various biological processes. Herein, we examined the role of exogenous Mg in augmenting wax gourd tolerance to salinity. The salinity-tolerant FenPi (FP) and sensitive YuXiang (YX) varieties were used in this study. The foliar spray of 25 mM Mg to FenPi (FP) and YuXiang (YX) line induced tolerance to 50 mM NaCl by enhancing the antioxidant activities such as SOD, POD, and CAT. The superoxide ion (O<sup>−2</sup>) level decreased, whereas the chlorophyll content and Fv/Fm values increased significantly under 25 mM Mg treatment. Additionally, 9 <i>BhMGT</i> were identified in the wax gourd genome. Various hormonal and stress-responsive <i>cis-</i>elements were recorded in the upstream region of <i>BhMGT</i> genes. The qRT-PCR expression analysis displayed a higher expression of <i>BhMGT1</i> and <i>BhMGT4</i> in the leaf tissue of FP than YX under 50 mM NaCl stress. Heterologous overexpression of <i>BhMGT1</i> and <i>BhMGT4</i> in yeast cells demonstrated vigorous growth activities in response to 50 mM NaCl stress. Our findings highlight the potential role of <i>BhMGT</i> genes in regulating the response of wax gourd to NaCl stress and provide a foundation/platform for future in planta research.</p>

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Magnesium Regulates Wax Gourd Salinity Response by Modulating Antioxidant Activities and MGT Expression

  • 期刊论文

  • 发表日期：

    2025年12月2日

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  • Shahid Hussain,
  • Jingjing Chang,
  • Jing Li,
  • Xiao Chen,
  • Lei Chen,
  • Zhao Song,
  • Baige Zhang

摘要

The magnesium transporter (MGT) gene family is known for transport activities and regulating responses to environmental stresses. Magnesium (Mg) is an essential nutrient required for various biological processes. Herein, we examined the role of exogenous Mg in augmenting wax gourd tolerance to salinity. The salinity-tolerant FenPi (FP) and sensitive YuXiang (YX) varieties were used in this study. The foliar spray of 25 mM Mg to FenPi (FP) and YuXiang (YX) line induced tolerance to 50 mM NaCl by enhancing the antioxidant activities such as SOD, POD, and CAT. The superoxide ion (O−2) level decreased, whereas the chlorophyll content and Fv/Fm values increased significantly under 25 mM Mg treatment. Additionally, 9 BhMGT were identified in the wax gourd genome. Various hormonal and stress-responsive cis-elements were recorded in the upstream region of BhMGT genes. The qRT-PCR expression analysis displayed a higher expression of BhMGT1 and BhMGT4 in the leaf tissue of FP than YX under 50 mM NaCl stress. Heterologous overexpression of BhMGT1 and BhMGT4 in yeast cells demonstrated vigorous growth activities in response to 50 mM NaCl stress. Our findings highlight the potential role of BhMGT genes in regulating the response of wax gourd to NaCl stress and provide a foundation/platform for future in planta research.