<p>Gibberellins (GAs) play a crucial role in plant growth, influencing processes such as cell elongation and cell wall development. However, their specific effects on the growing cell wall remain unclear. In this study, we investigated how GA regulates soybean (<i>Glycine max</i> L. Merrill) growth, with a particular focus on its impact on cell wall composition and carbohydrate metabolism. To achieve this, we conducted physiological, metabolic, and anatomical analyses. High GA levels promoted shoot elongation and lignin accumulation, while PAC treatment suppressed shoot growth and favored root biomass allocation and cellulose deposition. These architectural changes were accompanied by marked shifts in carbohydrate metabolism. Elevated GA decreased sucrose and increased fructose and glucose availability, likely through upregulation of invertase and sucrose synthase pathways. In contrast, GA-deficient plants accumulated sucrose and showed reduced sink strength. Photosynthetic efficiency and protein content were largely unaffected by GA modulation, suggesting that biomass partitioning and carbon allocation, rather than assimilation, drive the observed phenotypes. Cell wall analysis revealed a trade-off between cellulose and lignin content depending on GA status, with GA favoring lignification. Discrepancies between histochemical and biochemical data highlight tissue-specific responses in stems and leaves. Collectively, our results demonstrate that GA biosynthesis orchestrates growth, metabolism, and structural development in soybean. This has practical implications for breeding and agronomic strategies aimed at optimizing lodging tolerance, biomass quality, and resource use efficiency.</p>