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<p><i>TRANSPARENT TESTA GLABRA1</i> (TTG1)&#xa0;gene plays a crucial role in regulating the development of epidermal hairs in plants. Comparative sequencing and qRT-PCR analysis of the transcriptomes of the main cultivar of <i>Lonicera japonica</i> Thunb. (<i>L. japonica</i>) and the new cultivar of ‘Yujin 1’, characterized by dense and long epidermal hairs, revealed a significant upregulation in the expression level of <i>LjTTG1</i>. To investigate the role of the <i>LjTTG1</i> gene in the development of epidermal hairs in <i>L. japonica</i>, using ‘Yujin 1’ as the experimental material, <i>LjTTG1</i> gene was amplified by PCR with a full length of 1203&#xa0;bp. Subsequently, we performed bioinformatics analysis and Arabidopsis heterologous transformation. The expression of the transgenic <i>Arabidopsis thaliana LjTTG1</i> gene showed a significant increase, resulting in a 1.96-fold higher number of epidermal hairs on the first pair of true leaves compared to the wild type. This study successfully cloned the <i>LjTTG1</i> epidermal hair gene of <i>L. japonica</i> and verified its function in promoting epidermal hairs. This finding lays the foundation for further analyzing the molecular mechanism of epidermal hair development in <i>L. japonica</i>. In addition, it provides technical and theoretical support for molecular-assisted breeding of <i>L. japonica</i>, offering promising applications in the field.</p>

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Regulation of Epidermal Trichome Density in Arabidopsis thaliana and Tobacco Leaves by Overexpression of the LjTTG1 Gene from Lonicera japonica

  • 期刊论文

  • 发表日期：

    2025年7月17日

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  • Cuifang Chang,
  • Linrong Wang,
  • Bolun Yan,
  • Xinjie Yu,
  • Qian Huang,
  • Jianjun Li

摘要

TRANSPARENT TESTA GLABRA1 (TTG1) gene plays a crucial role in regulating the development of epidermal hairs in plants. Comparative sequencing and qRT-PCR analysis of the transcriptomes of the main cultivar of Lonicera japonica Thunb. (L. japonica) and the new cultivar of ‘Yujin 1’, characterized by dense and long epidermal hairs, revealed a significant upregulation in the expression level of LjTTG1. To investigate the role of the LjTTG1 gene in the development of epidermal hairs in L. japonica, using ‘Yujin 1’ as the experimental material, LjTTG1 gene was amplified by PCR with a full length of 1203 bp. Subsequently, we performed bioinformatics analysis and Arabidopsis heterologous transformation. The expression of the transgenic Arabidopsis thaliana LjTTG1 gene showed a significant increase, resulting in a 1.96-fold higher number of epidermal hairs on the first pair of true leaves compared to the wild type. This study successfully cloned the LjTTG1 epidermal hair gene of L. japonica and verified its function in promoting epidermal hairs. This finding lays the foundation for further analyzing the molecular mechanism of epidermal hair development in L. japonica. In addition, it provides technical and theoretical support for molecular-assisted breeding of L. japonica, offering promising applications in the field.