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<p>Volatile organic compounds derived from plant growth-promoting rhizobacteria play critical roles in enhancing plant growth and stress tolerance. This study evaluated the effects of S-methyl thioacetate (SMT) on tomato seedlings under hydroponic and soil conditions, complemented by transcriptomic analysis using RNA-Seq. Under 3&#xa0;g NaCl·kg<sup>−1</sup> soil conditions, 100&#xa0;mg·L<sup>−1</sup> SMT alleviated salt stress, enhancing shoot dry weight by 37.29% and root dry weight by 58.97%. In hydroponic experiments, 50&#xa0;mg·L<sup>−1</sup> SMT was optimal, increasing root and shoot dry weights by 79.18% and 62.64%, respectively, compared to the controls. This treatment also significantly increased chlorophyll content, root length, and root surface area by 22.64%, 34.65%, and 84.69%, respectively. RNA-Seq analyses of seedlings treated with 100&#xa0;mg·L<sup>−1</sup> SMT in hydroponic experiments identified 7,748 differentially expressed genes (DEGs). Gene ontology functional enrichment analysis revealed that DEGs were predominantly involved in biological processes related to fungi and immune response, molecular functions associated with ATP binding, protein serine/threonine kinase activity, protein kinase activity, cellular components linked to the plasma membrane, and integral membrane components. KEGG pathway enrichment analysis showed that SMT induced a genotype-dependent response pattern, with upregulated DEGs involved in glutathione metabolism, lignin biosynthesis, and plant-pathogen interaction pathways. Key upregulated genes included those encoding peroxidase, WRKY22/29, and HSP90. This study provides a mechanistic framework for SMT’s dual role in enhancing growth and stress resilience in tomato seedlings. Future field trials are essential to validate its agricultural applicability.</p>

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Transcriptome Analysis Reveals Impact of S-Methyl Thioacetate on Tomato Seedling Growth

  • 期刊论文

  • 发表日期：

    2025年6月21日

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  • Donghui Zheng,
  • Fangfang Ren,
  • Rongjun Yin,
  • Linmei Li,
  • Hanwen Cui,
  • Hui Shen,
  • Xiaochun Chen,
  • Nan Gao

摘要

Volatile organic compounds derived from plant growth-promoting rhizobacteria play critical roles in enhancing plant growth and stress tolerance. This study evaluated the effects of S-methyl thioacetate (SMT) on tomato seedlings under hydroponic and soil conditions, complemented by transcriptomic analysis using RNA-Seq. Under 3 g NaCl·kg−1 soil conditions, 100 mg·L−1 SMT alleviated salt stress, enhancing shoot dry weight by 37.29% and root dry weight by 58.97%. In hydroponic experiments, 50 mg·L−1 SMT was optimal, increasing root and shoot dry weights by 79.18% and 62.64%, respectively, compared to the controls. This treatment also significantly increased chlorophyll content, root length, and root surface area by 22.64%, 34.65%, and 84.69%, respectively. RNA-Seq analyses of seedlings treated with 100 mg·L−1 SMT in hydroponic experiments identified 7,748 differentially expressed genes (DEGs). Gene ontology functional enrichment analysis revealed that DEGs were predominantly involved in biological processes related to fungi and immune response, molecular functions associated with ATP binding, protein serine/threonine kinase activity, protein kinase activity, cellular components linked to the plasma membrane, and integral membrane components. KEGG pathway enrichment analysis showed that SMT induced a genotype-dependent response pattern, with upregulated DEGs involved in glutathione metabolism, lignin biosynthesis, and plant-pathogen interaction pathways. Key upregulated genes included those encoding peroxidase, WRKY22/29, and HSP90. This study provides a mechanistic framework for SMT’s dual role in enhancing growth and stress resilience in tomato seedlings. Future field trials are essential to validate its agricultural applicability.