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<p>Environmental conditions regulating secondary metabolites of plants have been extensively studied. However, the relationship between exogenous carbon (eCO<sub>2</sub>) and flavonoid biosynthesis of grape remains unrevealed, Hence, two-factor mixed experiments were designed, including CO<sub>2</sub> (ambient concentration: 380 ± 40&#xa0;μmol/mol; high concentration: 1000&#xa0;μmol/mol) and sucrose (0 and 20&#xa0;g/L). Four treatments were S1 (CK: ambient CO<sub>2</sub> with 20&#xa0;g/L sucrose), C0 (high CO<sub>2</sub> and no sucrose), S0 (ambient CO<sub>2</sub> and no sucrose) and Cs (high CO<sub>2</sub> with 20&#xa0;g/L sucrose). Subsequently, transcriptome and proteome analysis were performed on the experimental samples. The results revealed that 38 differentially expressed genes (DEGs, 14 up-regulated and 24 down-regulated) significantly differentially expressed in eCO<sub>2</sub>, which associated with flavonoid biosynthesis pathway. The up-regulated DEGs mainly include flavonoid-3, 5′-hydroxylase (<i>LOC100232896</i>, <i>LOC104877273</i>, <i>LOC100248071</i>), anthocyanin O-methyltransferase (<i>FAOMT</i>), chalcone synthase (<i>CHS3</i>, <i>CHS</i>), leucoanthocyanidin reductase 1 (<i>LAR1</i>), flavanone 3-hydroxylase (<i>F3H</i>), anthocyanidin reductase (<i>ANR</i>), leucoanthocyanidin dioxygenase (<i>LDOX</i>), dihydroflavonol reductase (<i>DFR</i>), chalcone isomerase (<i>CHI</i>), flavonoid 3′, 5′-methyltransferase (<i>LOC100245452</i>), and chalcone-flavonone isomerase 3 (<i>LOC100255217</i>). Moreover, there were three differentially expressed proteins (DEPs) involved in the flavonoid biosynthesis pathway, which were CHS, CHS3, and VIT_11s0037g00570. In conclusion, exogenous carbon maybe induce flavonoid metabolic pathway by promoting flavonoid metabolic enzyme expression to influence plant growth.</p>

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Flavonoid Metabolism Pathway Response to the Process of Exogenous Carbon Regulates Grape Growth

  • 期刊论文

  • 发表日期：

    2025年4月8日

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  • Han Wang,
  • Xin Zhao,
  • Zonghuan Ma,
  • Baihong Chen,
  • Juan Mao

摘要

Environmental conditions regulating secondary metabolites of plants have been extensively studied. However, the relationship between exogenous carbon (eCO2) and flavonoid biosynthesis of grape remains unrevealed, Hence, two-factor mixed experiments were designed, including CO2 (ambient concentration: 380 ± 40 μmol/mol; high concentration: 1000 μmol/mol) and sucrose (0 and 20 g/L). Four treatments were S1 (CK: ambient CO2 with 20 g/L sucrose), C0 (high CO2 and no sucrose), S0 (ambient CO2 and no sucrose) and Cs (high CO2 with 20 g/L sucrose). Subsequently, transcriptome and proteome analysis were performed on the experimental samples. The results revealed that 38 differentially expressed genes (DEGs, 14 up-regulated and 24 down-regulated) significantly differentially expressed in eCO2, which associated with flavonoid biosynthesis pathway. The up-regulated DEGs mainly include flavonoid-3, 5′-hydroxylase (LOC100232896, LOC104877273, LOC100248071), anthocyanin O-methyltransferase (FAOMT), chalcone synthase (CHS3, CHS), leucoanthocyanidin reductase 1 (LAR1), flavanone 3-hydroxylase (F3H), anthocyanidin reductase (ANR), leucoanthocyanidin dioxygenase (LDOX), dihydroflavonol reductase (DFR), chalcone isomerase (CHI), flavonoid 3′, 5′-methyltransferase (LOC100245452), and chalcone-flavonone isomerase 3 (LOC100255217). Moreover, there were three differentially expressed proteins (DEPs) involved in the flavonoid biosynthesis pathway, which were CHS, CHS3, and VIT_11s0037g00570. In conclusion, exogenous carbon maybe induce flavonoid metabolic pathway by promoting flavonoid metabolic enzyme expression to influence plant growth.