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<p>Biotic stress is a major limitation for productivity of different crop plants. To mitigate such stress, plants have evolved a plethora of mechanisms that finally result in enhanced plant fitness. Plant non-specific lipid transfer proteins (nsLTPs) are small, basic proteins known to play a crucial role in growth, development, and stress responses. To elucidate the mechanistic basis of resistance conferred by the <i>SlnsLTP</i> gene in tomato, we functionally characterized a <i>SlnsLTP</i> associated with induced resistance against diverse fungal pathogens. First, we examined the expression profile of <i>SlnsLTP</i> in response to phytohormones and pathogens, followed by analysis in a diverse set of hormone mutants and transgenics to understand its role in resistance. Additionally, we overexpressed the <i>SlnsLTP</i> gene in tomato and characterized the transgenic lines to further elucidate the mechanistic basis of resistance. Expression profile through quantitative real-time PCR revealed that <i>SlnsLTP</i> is upregulated in response to jasmonate (JA), abscisic acid (ABA) as well as pathogen inoculations and downregulated in mutants (or transgenics) altered in biosynthesis and/or signaling of diverse set of defense hormones. Alteration in expression of <i>SlnsLTP</i> in JA signaling mutant <i>jasmonic acid insensitive1 (jai1-1)</i> in response to with or without JA suggested that <i>SlnsLTP</i> is JA and JAI1 responsive. Further, our results demonstrated that overexpression of a <i>SlnsLTP</i> led to enhancement of resistance against foliar (<i>Botrytis cinerea</i>) and vascular (<i>Verticillium dahliae</i>) necrotrophic fungal pathogens. Expression analysis of salicylic acid (SA) and JA marker genes revealed the involvement of JA signaling pathway. Furthermore, we demonstrated that the level of acetoxytomatine, a potential antifungal specialized metabolite, accumulated in <i>SlnsLTP</i> overexpressing plants when challenged by the foliar pathogen <i>B. cinerea</i> but not by the vascular pathogen <i>V. dahliae</i>. Altogether, our findings demonstrated the crucial role of <i>SlnsLTP</i> in resistance against fungal pathogens, hence could be a potential candidate for improvement of crop plants against biotic stress.</p>

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Jasmonate Responsive SlnsLTP Confers Resistance Against Botrytis cinerea and Verticillium dahliae in Tomato

  • 期刊论文

  • 发表日期：

    2025年3月13日

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  • Sayantan Panda,
  • Namarta C. Singh,
  • Prashant D. Sonawane,
  • Sagit Meir,
  • Avinash C. Kamble

摘要

Biotic stress is a major limitation for productivity of different crop plants. To mitigate such stress, plants have evolved a plethora of mechanisms that finally result in enhanced plant fitness. Plant non-specific lipid transfer proteins (nsLTPs) are small, basic proteins known to play a crucial role in growth, development, and stress responses. To elucidate the mechanistic basis of resistance conferred by the SlnsLTP gene in tomato, we functionally characterized a SlnsLTP associated with induced resistance against diverse fungal pathogens. First, we examined the expression profile of SlnsLTP in response to phytohormones and pathogens, followed by analysis in a diverse set of hormone mutants and transgenics to understand its role in resistance. Additionally, we overexpressed the SlnsLTP gene in tomato and characterized the transgenic lines to further elucidate the mechanistic basis of resistance. Expression profile through quantitative real-time PCR revealed that SlnsLTP is upregulated in response to jasmonate (JA), abscisic acid (ABA) as well as pathogen inoculations and downregulated in mutants (or transgenics) altered in biosynthesis and/or signaling of diverse set of defense hormones. Alteration in expression of SlnsLTP in JA signaling mutant jasmonic acid insensitive1 (jai1-1) in response to with or without JA suggested that SlnsLTP is JA and JAI1 responsive. Further, our results demonstrated that overexpression of a SlnsLTP led to enhancement of resistance against foliar (Botrytis cinerea) and vascular (Verticillium dahliae) necrotrophic fungal pathogens. Expression analysis of salicylic acid (SA) and JA marker genes revealed the involvement of JA signaling pathway. Furthermore, we demonstrated that the level of acetoxytomatine, a potential antifungal specialized metabolite, accumulated in SlnsLTP overexpressing plants when challenged by the foliar pathogen B. cinerea but not by the vascular pathogen V. dahliae. Altogether, our findings demonstrated the crucial role of SlnsLTP in resistance against fungal pathogens, hence could be a potential candidate for improvement of crop plants against biotic stress.