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<p>The use of the entomopathogenic fungus <i>Metarhizium anisopliae</i> is an important strategy for green control of plant pests. <i>Metarhizium</i> can also act as a plant endophyte to promote plant growth. In this paper, we verified the endophyticity of <i>Metarhizium</i> in the root system of ‘Fengdan’ tree peony (<i>Paeonia ostii</i>) by various means. And the growth-promoting effect of <i>Metarhizium</i> on the growth of tree peony seedlings was explored. The results showed that <i>Metarhizium</i> spores were able to attach to the surface of the tree peony root system in large numbers after 12 h of treatment; mycelium could be observed to colonize the young root cells after 60 d of treatment, and the designed <i>Metarhizium</i> ITS-specific sequences were able to observe clear specific bands in the tree peony root system. The application of 1 × 10<sup>7</sup> spores·mL<sup>−1</sup> and 2 × 10<sup>7</sup> spores·mL<sup>−1</sup> <i>Metarhizium</i> treatment groups elevated root indole-3-acetic acid (IAA) content by 13.9% and 14.9% and brassinolide (BR) content by 11.0% and 20.6%. The expression of genes <i>YUCC10, CYP85A1</i>, and <i>DET2</i> related to endogenous hormone synthesis and genes <i>IAA11</i>, <i>IAA16</i>, <i>ARF5</i>, and <i>PID</i> related to the promotion of lateral root formation were significantly increased. (<i>p</i> &lt; 0.05). The net photosynthetic rate of leaves in the <i>Metarhizium</i> treatment group significantly increased. Additionally, there was a significant increase in the accumulation of soluble protein, soluble sugar, and starch in seedling roots, as well as in the number of lateral roots, root fresh mass, and single plant mass. The results suggested that <i>Metarhizium</i> was able to colonize the root system of tree peony to establish a symbiotic relationship, regulate the plant IAA and BR hormone signaling pathways and exhibit a significant growth-promoting effect.</p>

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Enhancing Seedling Growth and Root Development Through Symbiotic Interactions: The Role of Metarhizium anisopliae in Tree Peony Root Systems

  • 期刊论文

  • 发表日期：

    2025年2月27日

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  • Wenbin Zhou,
  • Linting Fan,
  • Shuangcheng Gao,
  • Shuang Zhou,
  • Guoan Shi

摘要

The use of the entomopathogenic fungus Metarhizium anisopliae is an important strategy for green control of plant pests. Metarhizium can also act as a plant endophyte to promote plant growth. In this paper, we verified the endophyticity of Metarhizium in the root system of ‘Fengdan’ tree peony (Paeonia ostii) by various means. And the growth-promoting effect of Metarhizium on the growth of tree peony seedlings was explored. The results showed that Metarhizium spores were able to attach to the surface of the tree peony root system in large numbers after 12 h of treatment; mycelium could be observed to colonize the young root cells after 60 d of treatment, and the designed Metarhizium ITS-specific sequences were able to observe clear specific bands in the tree peony root system. The application of 1 × 107 spores·mL−1 and 2 × 107 spores·mL−1 Metarhizium treatment groups elevated root indole-3-acetic acid (IAA) content by 13.9% and 14.9% and brassinolide (BR) content by 11.0% and 20.6%. The expression of genes YUCC10, CYP85A1, and DET2 related to endogenous hormone synthesis and genes IAA11, IAA16, ARF5, and PID related to the promotion of lateral root formation were significantly increased. (p < 0.05). The net photosynthetic rate of leaves in the Metarhizium treatment group significantly increased. Additionally, there was a significant increase in the accumulation of soluble protein, soluble sugar, and starch in seedling roots, as well as in the number of lateral roots, root fresh mass, and single plant mass. The results suggested that Metarhizium was able to colonize the root system of tree peony to establish a symbiotic relationship, regulate the plant IAA and BR hormone signaling pathways and exhibit a significant growth-promoting effect.