Histone deacetylase inhibition has been shown to modulate gene expression profiles in plants, thereby influencing the regulatory mechanisms involved in gametic embryogenesis. In this investigation, we examined the impact of various concentrations of trichostatin A (TSA), a potent inhibitor of histone deacetylases, on the androgenic response and the expression patterns of key genes associated with embryogenesis and histone deacetylation in anther -derived calli of Camelina sativa L through two separate experiments. Our findings revealed that TSA treatment of buds and also anther-derived calli significantly augmented embryogenesis in camelina anther cultures. The highest number of embryos (1.63 embryo per anther) was observed following pretreatment of buds with 2 μM TSA in the first experiment. Gene expression analysis indicated marked upregulation of BBM, LEC1, WUS, AGL15, and SERK genes, pivotal regulators implicated in the initiation of embryogenesis, in response to TSA treatments. The highest transcript accumulation for the LEC1, AGL15, and WUS genes was observed in calli derived from 2 μM TSA treated buds with expression levels 5.91-, 2.12-, and 2.08-fold higher than the control, respectively. Similarly, the application of both 1 μM and 2 μM TSA on the buds led to a significant increase in expression levels of BBM (9.58 and 11.16-fold higher than the control, respectively) and SERK (5.15- and 5.77-fold higher than the control, respectively). Furthermore, 2 μM TSA administration resulted in a significant increase in the expression levels of HDAC6 and HDAC19 genes (2- and 2.71- fold higher than control, respectively) in the first experiment. In second experiment, anther-derived calli treated with 2 μM TSA resulted to maximum percentage of embryogenic calli and mean embryo per callus (57.78% and 0.61 embryo per callus, respectively). In last experiment, transcript levels of BBM, WUS, AGL15, SERK, LEC1 and HDAC6 genes were found to be significantly elevated (9.42-, 3.90-, 3.35-, 5.60-, 19.16-, and twofold compared to controls, respectively) at a concentration of 2 μM TSA. Also, the expression levels of HDAC19 significantly enhanced at concentrations of 1 μM and 2 μM TSA, reaching 2.47 and 2.71-fold higher than controls, respectively. Statistical analysis showed a significant positive correlation (p < 0.01) between the expression profiles of embryogenesis genes and both the percentage of anthers producing calli and the average number of embryos per anther in the first experiment.

Epigenetic Regulation of Androgenic Responses in Camelina sativa L. Through Trichostatin A

期刊论文
发表日期：

2025年2月2日

查看全文

Zahra Behzadfar,
Mohammad Reza Abdollahi

摘要

Histone deacetylase inhibition has been shown to modulate gene expression profiles in plants, thereby influencing the regulatory mechanisms involved in gametic embryogenesis. In this investigation, we examined the impact of various concentrations of trichostatin A (TSA), a potent inhibitor of histone deacetylases, on the androgenic response and the expression patterns of key genes associated with embryogenesis and histone deacetylation in anther -derived calli of Camelina sativa L through two separate experiments. Our findings revealed that TSA treatment of buds and also anther-derived calli significantly augmented embryogenesis in camelina anther cultures. The highest number of embryos (1.63 embryo per anther) was observed following pretreatment of buds with 2 μM TSA in the first experiment. Gene expression analysis indicated marked upregulation of BBM, LEC1, WUS, AGL15, and SERK genes, pivotal regulators implicated in the initiation of embryogenesis, in response to TSA treatments. The highest transcript accumulation for the LEC1, AGL15, and WUS genes was observed in calli derived from 2 μM TSA treated buds with expression levels 5.91-, 2.12-, and 2.08-fold higher than the control, respectively. Similarly, the application of both 1 μM and 2 μM TSA on the buds led to a significant increase in expression levels of BBM (9.58 and 11.16-fold higher than the control, respectively) and SERK (5.15- and 5.77-fold higher than the control, respectively). Furthermore, 2 μM TSA administration resulted in a significant increase in the expression levels of HDAC6 and HDAC19 genes (2- and 2.71- fold higher than control, respectively) in the first experiment. In second experiment, anther-derived calli treated with 2 μM TSA resulted to maximum percentage of embryogenic calli and mean embryo per callus (57.78% and 0.61 embryo per callus, respectively). In last experiment, transcript levels of BBM, WUS, AGL15, SERK, LEC1 and HDAC6 genes were found to be significantly elevated (9.42-, 3.90-, 3.35-, 5.60-, 19.16-, and twofold compared to controls, respectively) at a concentration of 2 μM TSA. Also, the expression levels of HDAC19 significantly enhanced at concentrations of 1 μM and 2 μM TSA, reaching 2.47 and 2.71-fold higher than controls, respectively. Statistical analysis showed a significant positive correlation (p < 0.01) between the expression profiles of embryogenesis genes and both the percentage of anthers producing calli and the average number of embryos per anther in the first experiment.