Scholarlink

<p>Among diverse <i>Artemia</i> strains, Xizang <i>Artemia</i> exhibits higher baseline HUFA content, containing a rich eicosapentaenoic acid (EPA) and a minor amount of docosahexaenoic acid (DHA). Nevertheless, the content of DHA in Xizang <i>Artemia</i> remains insufficient for marine aquatic organisms, particularly marine fish. Moreover, the enrichment efficiency and metabolism of highly unsaturated fatty acid (HUFA) in Xizang <i>Artemia</i> are not yet fully elucidated. This research investigated the survival and HUFA metabolic rates of <i>Artemia tibetiana</i> during the enrichment process with DHA-rich emulsified fish oil and in the subsequent 6, 12, 24, 48, and 72 h starvation at 14, 19, and 24 °C, in comparison with <i>Artemia franciscana</i>. The findings indicated that <i>A. tibetiana</i> achieved the highest DHA content of 9.78 mg/g dry weight (DW) after 36-h enrichment, whereas <i>A. franciscana</i> reached its peak DHA content of 31.40 mg/g DW after 24-h enrichment. After 72-h starvation, the DHA content in both species declined rapidly, with a metabolic rate exceeding 70%. Both species exhibited relatively lower metabolic rates for EPA and arachidonic acid (ARA), less than 50% and 38% respectively. After 72-h starvation, the DHA metabolic rates of <i>A. tibetiana</i> were significantly lower than those of <i>A. franciscana</i> at the three temperatures (<i>P</i>&lt; 0.05), demonstrating species-specific characteristics. Two-factor analysis of variance revealed that the metabolic rates of ARA, EPA, and DHA increased significantly with the increased starvation temperature and the prolonged starvation time (<i>P</i>&lt;0.001). In conclusion, the starting enrichment time and enrichment duration should be determined according to the <i>Artemia</i> species/strains in order to guarantee the enrichment efficiency. Additionally, the remaining HUFA-enriched <i>Artemia</i> should be stored at a lower temperature to minimize the metabolic consumption of HUFA and ensure a higher survival rate. This study offers a reference for studying the HUFA metabolic pattern of <i>Artemia</i> and provides guidance for the application of <i>Artemia</i> enrichment and the preservation of HUFA-enriched <i>Artemia</i> in hatcheries.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

HUFA metabolism patterns of brine shrimp Artemia tibetiana at different temperatures

  • 期刊论文

  • 发表日期：

    2026年6月29日

查看全文
  • Wei Xie,
  • Honggang Deng,
  • Yulong Lu,
  • Meirong Gao,
  • Zhenqian Wang,
  • Kai He,
  • Hongchi Li,
  • Kaiyu Zhao,
  • Liying Sui,
  • Chi Zhang

摘要

Among diverse Artemia strains, Xizang Artemia exhibits higher baseline HUFA content, containing a rich eicosapentaenoic acid (EPA) and a minor amount of docosahexaenoic acid (DHA). Nevertheless, the content of DHA in Xizang Artemia remains insufficient for marine aquatic organisms, particularly marine fish. Moreover, the enrichment efficiency and metabolism of highly unsaturated fatty acid (HUFA) in Xizang Artemia are not yet fully elucidated. This research investigated the survival and HUFA metabolic rates of Artemia tibetiana during the enrichment process with DHA-rich emulsified fish oil and in the subsequent 6, 12, 24, 48, and 72 h starvation at 14, 19, and 24 °C, in comparison with Artemia franciscana. The findings indicated that A. tibetiana achieved the highest DHA content of 9.78 mg/g dry weight (DW) after 36-h enrichment, whereas A. franciscana reached its peak DHA content of 31.40 mg/g DW after 24-h enrichment. After 72-h starvation, the DHA content in both species declined rapidly, with a metabolic rate exceeding 70%. Both species exhibited relatively lower metabolic rates for EPA and arachidonic acid (ARA), less than 50% and 38% respectively. After 72-h starvation, the DHA metabolic rates of A. tibetiana were significantly lower than those of A. franciscana at the three temperatures (P< 0.05), demonstrating species-specific characteristics. Two-factor analysis of variance revealed that the metabolic rates of ARA, EPA, and DHA increased significantly with the increased starvation temperature and the prolonged starvation time (P<0.001). In conclusion, the starting enrichment time and enrichment duration should be determined according to the Artemia species/strains in order to guarantee the enrichment efficiency. Additionally, the remaining HUFA-enriched Artemia should be stored at a lower temperature to minimize the metabolic consumption of HUFA and ensure a higher survival rate. This study offers a reference for studying the HUFA metabolic pattern of Artemia and provides guidance for the application of Artemia enrichment and the preservation of HUFA-enriched Artemia in hatcheries.