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<p>Glucose homeostasis is a fundamental physiological process in both vertebrates and invertebrates, yet its regulatory mechanisms in molluscs remain largely unexplored. This study investigates temporal hemolymph glucose dynamics and associated molecular responses in the Pacific oyster <i>Crassostrea gigas</i>. Annual monitoring revealed significant seasonal variation in hemolymph glucose concentrations, with post-spawning oysters exhibiting the lowest levels. A glucose injection experiment demonstrated rapid uptake kinetics, followed by a return to baseline, suggesting the presence of efficient metabolic regulation. Transcriptomic analysis on hepatopancreas tissue identified <i>cgHK2-2</i> as the dominant hexokinase isoform induced by hyperglycemia, while other <i>HK</i> genes (<i>cgHK2</i> and <i>cgHK2-like</i>) showed tissue-specific but non-inducible expression profiles in the investigated tissue. Furthermore, upregulation of <i>cgPPP1R3B</i> and <i>cgPCSK1</i> indicate possibly conserved glycogen metabolism and insulinlike signaling pathways. Phylogenetic analysis revealed divergent evolutionary trajectories of hexokinase in protostomes versus chordates, with oysters lacking a clear glucokinase orthologue. These findings highlight key molecular players in oyster glucose metabolism and suggest both conserved and lineage-specific regulatory strategies.</p>

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Molecular basis of glucose homeostasis in the Pacific oyster (Crassostrea gigas): insights from transcriptome and hexokinase phylogeny analysis

  • 期刊论文

  • 发表日期：

    2025年12月16日

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  • Zhitong Liu,
  • Xiaojing Miao,
  • Fei Xu

摘要

Glucose homeostasis is a fundamental physiological process in both vertebrates and invertebrates, yet its regulatory mechanisms in molluscs remain largely unexplored. This study investigates temporal hemolymph glucose dynamics and associated molecular responses in the Pacific oyster Crassostrea gigas. Annual monitoring revealed significant seasonal variation in hemolymph glucose concentrations, with post-spawning oysters exhibiting the lowest levels. A glucose injection experiment demonstrated rapid uptake kinetics, followed by a return to baseline, suggesting the presence of efficient metabolic regulation. Transcriptomic analysis on hepatopancreas tissue identified cgHK2-2 as the dominant hexokinase isoform induced by hyperglycemia, while other HK genes (cgHK2 and cgHK2-like) showed tissue-specific but non-inducible expression profiles in the investigated tissue. Furthermore, upregulation of cgPPP1R3B and cgPCSK1 indicate possibly conserved glycogen metabolism and insulinlike signaling pathways. Phylogenetic analysis revealed divergent evolutionary trajectories of hexokinase in protostomes versus chordates, with oysters lacking a clear glucokinase orthologue. These findings highlight key molecular players in oyster glucose metabolism and suggest both conserved and lineage-specific regulatory strategies.