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<p>Alternative splicing (AS) is a fundamental cellular process that enables organisms to adapt effectively to diverse stressors. However, the contribution of AS in salinity response has not been reported in crab. We examined the AS events in the mud crab, <i>Scylla paramamosain</i>, a euryhaline marine crab by RNA-Seq. More AS events were identified under common salinity (23) than that of under low salinity (5). Furthermore, more differential alternative splicing (DAS) events between low and common salinity were characterized in eyestalk (1 037) than those in gill (512) and antennal gland (413), respectively. Functional enrichment analysis revealed tissue-specific pathways, with DAS genes (DASGs) in eyestalk associated with upstream signal transduction, while those in gill and antennal gland were linked to energy metabolism and absorption functions. The limited overlap between DASGs and differentially expressed genes (DEGs) suggested independent regulation of AS and transcription. Importantly, functional enrichment results highlighted that DASGs were enriched in energy metabolism and signal transduction, whereas DEGs were enriched in transporter and metabolic pathways. The expression of splicing factors (SFs) revealed tissue-specific clustering and self-splicing characteristics, suggesting that SFs influenced downstream pathways by self-splicing. This study sheds lights into the regulatory dynamics of AS in crustacean low salinity adaptation.</p>

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Alternative splicing genes/events play important role in adaptation of mud crab Scylla paramamosain to low salinity

  • 期刊论文

  • 发表日期：

    2025年12月8日

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  • Shucheng Shao,
  • Nan Mo,
  • Chenchang Bao,
  • Zhaoxia Cui

摘要

Alternative splicing (AS) is a fundamental cellular process that enables organisms to adapt effectively to diverse stressors. However, the contribution of AS in salinity response has not been reported in crab. We examined the AS events in the mud crab, Scylla paramamosain, a euryhaline marine crab by RNA-Seq. More AS events were identified under common salinity (23) than that of under low salinity (5). Furthermore, more differential alternative splicing (DAS) events between low and common salinity were characterized in eyestalk (1 037) than those in gill (512) and antennal gland (413), respectively. Functional enrichment analysis revealed tissue-specific pathways, with DAS genes (DASGs) in eyestalk associated with upstream signal transduction, while those in gill and antennal gland were linked to energy metabolism and absorption functions. The limited overlap between DASGs and differentially expressed genes (DEGs) suggested independent regulation of AS and transcription. Importantly, functional enrichment results highlighted that DASGs were enriched in energy metabolism and signal transduction, whereas DEGs were enriched in transporter and metabolic pathways. The expression of splicing factors (SFs) revealed tissue-specific clustering and self-splicing characteristics, suggesting that SFs influenced downstream pathways by self-splicing. This study sheds lights into the regulatory dynamics of AS in crustacean low salinity adaptation.