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<p><i>Ostreopsis</i> cf. <i>ovata</i> is a marine benthic dinoflagellate in tropical and temperate seas and can produce potent toxic compounds. The existence of <i>O.</i> cf. <i>ovata</i> has been found in the Chinese coastal areas, but studies on its toxicity are very few. This study investigated the toxicity of the <i>O.</i> cf. <i>ovata</i> (TIO991) isolated from Weizhou Island in the South China Sea by using methanol and chloroform to extract toxic compounds from the algal cells cultured indoor. Experiments on mouse acute toxicity showed that the crude methanol extract (CME) of <i>O.</i> cf. <i>ovata</i> caused the death of mice in 16–18 min. Furthermore, CME inhibited the cell reproduction of human neuroblastoma cells (BE (2)-M17 cells) by Cell Counting Kit-8 with a dose- and time-effect relationship and caused cell death in the form of cell necrosis. We found that CME had strong hemolytic activity and was significantly inhibited by ouabain, indicating that CME might contain palytoxins. By contrast, the crude chloroform extract of <i>O.</i> cf. <i>ovata</i> was relatively weak in toxicity as obtained in our experiments on mouse acute toxicity, cytotoxicity, and hemolytic activity. This suggests that the algae may raise the potential threat to marine ecosystems and public health.</p>

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Toxicity testing of Ostreopsis cf. ovata: mouse bioassay, cytotoxicity, and hemolysis neutralization assay

  • 期刊论文

  • 发表日期：

    2025年4月25日

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  • Yuping Lai,
  • Qingchun Xu,
  • Wenyu Gan,
  • Hanbai Wang,
  • Shiwei Kou,
  • Tianjiu Jiang,
  • Tao Jiang

摘要

Ostreopsis cf. ovata is a marine benthic dinoflagellate in tropical and temperate seas and can produce potent toxic compounds. The existence of O. cf. ovata has been found in the Chinese coastal areas, but studies on its toxicity are very few. This study investigated the toxicity of the O. cf. ovata (TIO991) isolated from Weizhou Island in the South China Sea by using methanol and chloroform to extract toxic compounds from the algal cells cultured indoor. Experiments on mouse acute toxicity showed that the crude methanol extract (CME) of O. cf. ovata caused the death of mice in 16–18 min. Furthermore, CME inhibited the cell reproduction of human neuroblastoma cells (BE (2)-M17 cells) by Cell Counting Kit-8 with a dose- and time-effect relationship and caused cell death in the form of cell necrosis. We found that CME had strong hemolytic activity and was significantly inhibited by ouabain, indicating that CME might contain palytoxins. By contrast, the crude chloroform extract of O. cf. ovata was relatively weak in toxicity as obtained in our experiments on mouse acute toxicity, cytotoxicity, and hemolytic activity. This suggests that the algae may raise the potential threat to marine ecosystems and public health.