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<p>5-hydroxytryptamine (5-HT) is a biological monoamine neurotransmitter in the central nervous system and gonads of crustaceans to induce gonadal maturation. To better understand the effects of 5-HT and its receptors on reproductive development in <i>Artemia</i>, a 5-HT type 7 receptor gene (<i>5-HT</i><sub><i>7Ar</i></sub>) was identified in parthenogenetic <i>Artemia</i> and characterized. Sequence analysis revealed that the open reading frame of <i>5-HT</i><sub><i>7Ar</i></sub> encodes a 414-aa protein. <i>5-HT</i><sub><i>7Ar</i></sub> showed higher expression in both brain and ovary at early embryo stage, <i>5-HT</i><sub><i>7Ar</i></sub> could be detected during ovarian development and the highest expression was observed at early embryo stage. Silencing of the <i>5-HT</i><sub><i>7Ar</i></sub> in <i>Artemia</i> at early embryo stage decreased significantly the expression level of <i>5-HT</i><sub><i>7Ar</i></sub> gene and protein at late oocyte, early embryo, and late embryo stage. Moreover, silencing of the <i>5-HT</i><sub><i>7Ar</i></sub> resulted in a decreased fecundity, which corresponds to abnormal oocytes during the embryo development. <i>Artemia</i> tended to produce nauplii after the silencing of <i>5-HT</i><sub><i>7Ar</i></sub>, indicating that <i>5-HT</i><sub><i>7Ar</i></sub> may also involve in the determination of its reproduction mode. The findings of this study provide an insight into the regulation of reproductive development in <i>Artemia</i> and the function of <i>5-HT</i><sub><i>7Ar</i></sub>.</p>

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Effect of the 5-HT7Ar gene on the reproductive performance of parthenogenetic Artemia

  • 期刊论文

  • 发表日期：

    2025年5月22日

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  • Rui Zhang,
  • Xuemei Ouyang,
  • Ke Li,
  • Chi Zhang,
  • Liangsen Liu,
  • Liying Sui,
  • Xuekai Han

摘要

5-hydroxytryptamine (5-HT) is a biological monoamine neurotransmitter in the central nervous system and gonads of crustaceans to induce gonadal maturation. To better understand the effects of 5-HT and its receptors on reproductive development in Artemia, a 5-HT type 7 receptor gene (5-HT7Ar) was identified in parthenogenetic Artemia and characterized. Sequence analysis revealed that the open reading frame of 5-HT7Ar encodes a 414-aa protein. 5-HT7Ar showed higher expression in both brain and ovary at early embryo stage, 5-HT7Ar could be detected during ovarian development and the highest expression was observed at early embryo stage. Silencing of the 5-HT7Ar in Artemia at early embryo stage decreased significantly the expression level of 5-HT7Ar gene and protein at late oocyte, early embryo, and late embryo stage. Moreover, silencing of the 5-HT7Ar resulted in a decreased fecundity, which corresponds to abnormal oocytes during the embryo development. Artemia tended to produce nauplii after the silencing of 5-HT7Ar, indicating that 5-HT7Ar may also involve in the determination of its reproduction mode. The findings of this study provide an insight into the regulation of reproductive development in Artemia and the function of 5-HT7Ar.